|Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. GeneTex’s proTUNEL-Red In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells. |
‧ Detection method- Flow cytometry (Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD).
‧ Sample type- Tissue sections and fixed cell preparations
‧ Species reactivity- Mammalian
‧ Kit size- 60 assays
‧ Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation
Features and Benefits
‧ Simple procedure; takes ~ 3 hours
‧ Fast and convenient
‧ The assay is sensitive and stable
‧ The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Positive Control Cells
Negative Control Cells
Anti-BrdU-Red Mouse IgG1
7-AAD/RNase Staining Buffer