Catalog Number
|
GTX70228

(
101
)
|
Product Name
|
Rad50 antibody [13B3]
|
Applications | ICC/IF, IHC, IHC-P, IP, WB, in vitro |
Application Note |
| Suggested dilution | Reference |
Immunocytochemistry/ Immunofluorescence (ICC/IF) | 1:100-1:1000* |
Y |
Immunohistochemistry (IHC) | Assay-dependent dilution |
Y |
IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
|
Immunoprecipitation | Assay-dependent dilution |
|
Western Blot (WB) | 1:500-1:3000* |
Y |
in vitro assay | Assay-dependent dilution |
Y |
Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.
|
Predicted Target Size | 153 kDa (note)
|
Positive Controls | HeLa , HeLa nuclear extract |
Form Supplied | Liquid |
Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
Purification | Affinity purified by Protein G. |
Full Name | RAD50 homolog (S. cerevisiae) |
Background | RAD50 is an essential gene for all cell types and the expression does not vary during cell cycle. It is an excellent loading control at the high molecular weight position (150KD), complementing GAPDH, beta-Actin and alpha-Tubulin at the lower molecular weight positions. |
Synonyms | RAD50-2, hRad50, RAD50, 10111, RAD502, Q92878, 604040, NBSLD |
Cellular Localization | Nuclear |
Host | Mouse |
Clonality | Monoclonal |
Clone Name | 13B3 |
Isotype | IgG1 |
Immunogen | Amino acids 1-425 of Rad50 expressed in E. coli. |
Light Chain | kappa |
Antigen Species | Human |
Species Reactivity | Human, Mouse, Monkey, Rat |
Conjugation | Unconjugated |