|
Catalog Number
|
GTX70228
(
0
)
(
77
)
|
|
Product Name
|
Rad50 antibody [13B3]
|
| Full Name | RAD50 homolog (S. cerevisiae) |
| Synonyms | RAD50-2, hRad50, RAD50, 10111, RAD502, Q92878, 604040, NBSLD |
| Product Description | Mouse monoclonal [13B3] to Rad50 |
| Background | RAD50 is an essential gene for all cell types and the expression does not vary during cell cycle. It is an excellent loading control at the high molecular weight position (150KD), complementing GAPDH, beta-Actin and alpha-Tubulin at the lower molecular weight positions. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Light Chain | kappa |
| Target | Rad50 |
| Immunogen | Amino acids 1-425 of Rad50 expressed in E. coli. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Monkey, Rat |
| Applications | ICC/IF, IHC, IP, WB |
| Application Note | For IP: Use at an assay dependent dilution. Suggested working dilution for GTX70228 for western blot: 1:500-3000. Immunofluorescence: 1:5000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Positive Controls | Raji , HeLa |
| Predicted Target Size | 153 |
| Cellular Localization | Nuclear |
| Form Supplied | Liquid |
| Purification | Protein G Affinity Purified |
| Purification Note | From ascitic fluid |
| Concentration | 1
mg/ml
|
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| ResearchArea | |
|