Rad50 antibody [13B3]

Knockout/Knockdown validated

Host / ClonalityMouse Monoclonal
Clone Name13B3
ApplicationsICC/IF, IHC, IHC-P, IP, WB
Species reactivityHuman, Mouse, Monkey, Rat
( 90 )

Cat No:

GTX70228

Package	Price	Qty
100 μl	USD 299.00

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Reference (90)

Application Information: Rad50 antibody [13B3]

Catalog Number

GTX70228

Product Name

Rad50 antibody [13B3]

See all Rad50 products

Applications

ICC/IF, IHC, IHC-P, IP, WB

Application Note

	Suggested dilution	Reference
ICC/IF	1:100-1:1000*	Y
Immunohistochemistry	Assay-dependent dilution	Y
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Immunoprecipitation	Assay-dependent dilution
Western blot	1:500-1:3000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

153 kDa (note)

Positive Controls

HeLa , Raji

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Protein G Affinity Purified

Purification Note

From ascitic fluid

Specifications: Rad50 antibody [13B3]

Full Name	RAD50 homolog (S. cerevisiae)
Product Description	Mouse monoclonal [13B3] to Rad50
Background	RAD50 is an essential gene for all cell types and the expression does not vary during cell cycle. It is an excellent loading control at the high molecular weight position (150KD), complementing GAPDH, beta-Actin and alpha-Tubulin at the lower molecular weight positions.
Synonyms	RAD50-2, hRad50, RAD50, 10111, RAD502, Q92878, 604040, NBSLD
Cellular Localization	Nuclear
Host	Mouse
Clonality	Monoclonal
Clone Name	13B3
Target	Rad50
Isotype	IgG1
Immunogen	Amino acids 1-425 of Rad50 expressed in E. coli.
Light Chain	kappa
Antigen Species	Human
Species Reactivity	Human, Mouse, Monkey, Rat

Storage Conditions: Rad50 antibody [13B3]

Storage Buffer	Phosphate-buffered saline, pH 7.4, containing no preservatives
Storage Instruction	Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Type of cancer > Breast > Other Cancer > Type of cancer > Ovarian Cancer > Type of cancer > Urogenital > Urinary Bladder Cell Biology > Cell cycle More Hide

Rad50 antibody [13B3] validated data

GTX70228 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500.

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GTX70228 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX70228) diluted at 1:500.

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GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in human lung by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Top

GTX70228 WB Image

Rad50 antibody [13B3] detects Rad50 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
Rad50 antibody [13B3] (GTX70228) dilution: 1:1000

Top

GTX70228 ICC/IF Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

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GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Top

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Top

GTX70228 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500.

Top

GTX70228 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX70228) diluted at 1:500.

Top

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in human lung by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Top

GTX70228 WB Image

Rad50 antibody [13B3] detects Rad50 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
Rad50 antibody [13B3] (GTX70228) dilution: 1:1000

Top

GTX70228 ICC/IF Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Top

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Top

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Top

Reference for Rad50 antibody [13B3]

Application References	Application	Reactivity
Lee KY et al. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. Nat Commun 6:7744 (2015).
Xiong J et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. J Cell Biol 208 (5):513-20 (2015).
Virts EL et al. AluY-mediated germline deletion, duplication and somatic stem cell reversion in UBE2T defines a new subtype of Fanconi anemia. Hum Mol Genet 24 (18):5093-108 (2015).	WB	Human
Harten SK et al. The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers. BMC Biol 13:21 (2015).	WB	Mouse
Kinoshita E et al. Human RAD50 makes a functional DNA-binding complex. Biochimie (2015).	WB	Human
Huang YL et al. FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis. Hum Mol Genet. 24:3506-17 (2015).	WB	Human
Sanjiv K et al. PI3K inhibition augments the therapeutic efficacy of a 3a-aza-cyclopenta[α]indene derivative in lung cancer cells Translational Oncology (2014).	WB	Human
Ohara M et al. Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination. J Radiat Res (2014).	WB	Human
Tahara M et al. The use of olaparib (AZD2281) potentiates SN-38 cytotoxicity in colon cancer cells by indirect inhibition of Rad51-mediated repair of DNA double-strand breaks. Mol Cancer Ther (2014).	WB	Human
Kong X et al. Distinct Functions of Human Cohesin-SA1 and Cohesin-SA2 in Double-Strand Break Repair. Mol Cell Biol 34 (4):685-98 (2014).	IP, WB	Human
Bohringer M et al. siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors. Oncogene 32 (48):5458-70 (2013).
Simoes M et al. Host DNA damage response facilitates African swine fever virus infection. Vet Microbiol 165 (1-2):140-7 (2013).	WB
Lee JH et al. Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex. J Biol Chem 288 (18):12840-51 (2013).	WB
Zhu J et al. A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia. EMBO Mol Med 5 (3):353-65 (2013).	WB	Human
Floyd SR et al. The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. Nature 498 (7453):246-50 (2013).
Oplustilova L et al. Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment. Cell Cycle 11 (20):3837-50 (2012).	WB	Human
Ogiwara H et al. CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes. PLoS One 7 (12):e52810 (2012).	WB	Human
Knies K et al. Genotyping of fanconi anemia patients by whole exome sequencing: advantages and challenges. PLoS One 7 (12):e52648 (2012).	WB	Human
Matsumoto Y et al. Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly. DNA Repair (Amst) 10 (3):314-21 (2011).	WB	Human
Masuda Y et al. Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin. Biochem Biophys Res Commun 415 (2):305-12 (2011).	WB	Human
Cheng Q et al. Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res 39 (22):9605-19 (2011).	ICC/IF
Baydoun HH et al. Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair. Blood 117 (22):5897-906 (2011).	ICC/IF	Human
Stoepker C et al. SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype. Nat Genet 43 (2):138-41 (2011).
Luo Y et al. Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death. J Virol 85 (1):133-45 (2011).
Anderson VE et al. CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res 71 (2):463-72 (2011).
Machida K et al. Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes. J Immunol 185 (11):6985-98 (2010).	WB
Lee JH et al. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J 29 (3):574-85 (2010).	WB
Palagyi A et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer 9:127 (2010).	WB	Human
Chailleux C et al. Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex. Biochem J 426 (3):365-71 (2010).	WB	Human
Momcilovic O et al. DNA damage responses in human induced pluripotent stem cells and embryonic stem cells. PLoS One 5 (10):e13410 (2010).	WB	Human
Balasubramanian N et al. Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex. J Virol 84 (24):12504-14 (2010).	ICC/IF
Cha H et al. Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response. Cancer Res 70 (10):4112-22 (2010).	ICC/IF	Human
Gomez-Godinez V et al. Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation. Nucleic Acids Res 38 (22):e202 (2010).
Kuroda S et al. Telomerase-dependent oncolytic adenovirus sensitizes human cancer cells to ionizing radiation via inhibition of DNA repair machinery. Cancer Res 70 (22):9339-48 (2010).
Schwartz RA et al. Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase. J Virol 83 (12):6269-78 (2009).	WB	Human
Moody CA et al. Human papillomaviruses activate the ATM DNA damage pathway for viral genome amplification upon differentiation. PLoS Pathog 5 (10):e1000605 (2009).	WB	Human
Collaco RF et al. Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways. Virology 392 (1):24-33 (2009).	ICC/IF	Human
Waltes R et al. Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder. Am J Hum Genet 84 (5):605-16 (2009).	ICC/IF	Human
Zheng L et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res 37 (8):2645-57 (2009).		Human
Carson CT et al. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection. EMBO J 28 (6):652-62 (2009).		Human
Gregory DA et al. Characterization of mre11 loss following HSV-1 infection. Virology 373 (1):124-36 (2008).	WB	Human
Schwartz RA et al. Distinct requirements of adenovirus E1b55K protein for degradation of cellular substrates. J Virol 82 (18):9043-55 (2008).	WB	Human
Bartkova J et al. Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene. Mol Oncol 2 (4):296-316 (2008).	ICC/IF	Human
Cervelli T et al. Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins. J Cell Sci 121 (Pt 3):349-57 (2008).
Lakdawala SS et al. Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation. J Virol 82 (17):8362-72 (2008).		Human
You KT et al. Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. PLoS Biol 5 (5):e109 (2007).	WB
Zhong ZH et al. Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres. J Biol Chem 282 (40):29314-22 (2007).	WB
Lin X et al. RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival. Mol Cell Biol 27 (13):4905-16 (2007).	WB	Human
Bae NS et al. A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends. Mol Cell 26 (3):323-34 (2007).	WB	Human
Bentle MS et al. Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone. Cancer Res 67 (14):6936-45 (2007).	ICC/IF	Human
Schwartz RA et al. The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. J Virol 81 (23):12936-45 (2007).
Cho YW et al. PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J Biol Chem 282 (28):20395-406 (2007).
Fleisig HB et al. Adenoviral E1B55K oncoprotein sequesters candidate leukemia suppressor sequence-specific single-stranded DNA-binding protein 2 into aggresomes. Oncogene 26 (33):4797-805 (2007).
Potts PR et al. The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins. Nat Struct Mol Biol 14 (7):581-90 (2007).
Liu L et al. Telomere lengthening early in development. Nat Cell Biol 9 (12):1436-41 (2007).
Tommiska J et al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer 118 (11):2911-6 (2006).	WB
Takemura H et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem 281 (41):30814-23 (2006).	WB	Human
Oishi H et al. An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification. J Biol Chem 281 (1):20-6 (2006).	WB	Human
Kong LJ et al. The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1. Mol Cell 22 (1):63-71 (2006).	WB	Human
Dote H et al. Inhibition of hsp90 compromises the DNA damage response to radiation. Cancer Res 66 (18):9211-20 (2006).	ICC/IF	Human
Mongiat-Artus P et al. Microsatellite instability and mutation analysis of candidate genes in urothelial cell carcinomas of upper urinary tract. Oncogene 25 (14):2113-8 (2006).	IHC	Human
Myers JS et al. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J Biol Chem 281 (14):9346-50 (2006).	IHC	Human
van der Burg M et al. A new type of radiosensitive T-B-NK+ severe combined immunodeficiency caused by a LIG4 mutation. J Clin Invest 116 (1):137-45 (2006).
Koh KH et al. Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas. Lab Invest 85 (9):1130-8 (2005).	WB	Human
van Veelen LR et al. Analysis of ionizing radiation-induced foci of DNA damage repair proteins. Mutat Res 574 (1-2):22-33 (2005).	ICC/IF
Fasching CL et al. Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies. Cancer Res 65 (7):2722-9 (2005).	ICC/IF
Kim JS et al. Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells. J Cell Biol 170 (3):341-7 (2005).	ICC/IF	Human
Liu Y et al. Adenovirus exploits the cellular aggresome response to accelerate inactivation of the MRN complex. J Virol 79 (22):14004-16 (2005).	ICC/IF	Human
Lilley CE et al. DNA repair proteins affect the lifecycle of herpes simplex virus 1. Proc Natl Acad Sci U S A 102 (16):5844-9 (2005).		Human
Dork T et al. Slow progression of ataxia-telangiectasia with double missense and in frame splice mutations. Am J Med Genet A 126A (3):272-7 (2004).	WB
O'Connor MS et al. The human Rap1 protein complex and modulation of telomere length. J Biol Chem 279 (27):28585-91 (2004).	WB	Human
Giannini G et al. Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability. Oncogene 23 (15):2640-7 (2004).	WB	Human
Angele S et al. Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast. J Clin Pathol 57 (11):1179-84 (2004).	IHC	Human
Wilkinson DE et al. Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response. J Virol 78 (9):4783-96 (2004).
Anderson LA et al. Regulation of RelA (p65) function by the large subunit of replication factor C. Mol Cell Biol 23 (2):721-32 (2003).	WB	Human
Xu X et al. NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors. FASEB J 17 (13):1842-8 (2003).	WB	Human
Carson CT et al. The Mre11 complex is required for ATM activation and the G2/M checkpoint. EMBO J 22 (24):6610-20 (2003).	WB	Human
Hassane DC et al. Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells. Infect Immun 71 (1):541-5 (2003).	ICC/IF	Human
Uziel T et al. Requirement of the MRN complex for ATM activation by DNA damage. EMBO J 22 (20):5612-21 (2003).	ICC/IF	Human
Angele S et al. Altered expression of DNA double-strand break detection and repair proteins in breast carcinomas. Histopathology 43 (4):347-53 (2003).	IHC	Human
Stewart GS et al. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature 421 (6926):961-6 (2003).
Pichierri P et al. DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein. Hum Mol Genet 11 (21):2531-46 (2002).	ICC/IF
Franchitto A et al. Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest. J Cell Biol 157 (1):19-30 (2002).	ICC/IF
Kim JS et al. Specific recruitment of human cohesin to laser-induced DNA damage. J Biol Chem 277 (47):45149-53 (2002).	ICC/IF	Human
Richie CT et al. hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage. Mol Cell Biol 22 (24):8635-47 (2002).	ICC/IF	Human
Stracker TH et al. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature 418 (6895):348-52 (2002).	ICC/IF	Human
Tauchi H et al. The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50[middle dot]hMRE11[middle dot]NBS1 complex DNA repair activity. J Biol Chem 276 (1):12-5 (2001).	ICC/IF	Human
Harfst E et al. Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome. Mol Immunol 37 (15):915-29 (2000).	WB
Wang Y et al. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev 14 (8):927-39 (2000).	ICC/IF	Human
Paull TT et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol 10 (15):886-95 (2000).	ICC/IF	Human

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Rad50 antibody [13B3]

Rad50 antibody [13B3]

Application Information: Rad50 antibody [13B3]

Specifications: Rad50 antibody [13B3]

Storage Conditions: Rad50 antibody [13B3]