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CD45RA antibody [GT0014]

Anti-CD45RA antibody [GT0014] used in Flow cytometry (FACS). GTX628507
Anti-CD45RA antibody [GT0014] used in Western Blot (WB). GTX628507
Anti-CD45RA antibody [GT0014] used in Flow cytometry (FACS). GTX628507
Anti-CD45RA antibody [GT0014] used in Flow cytometry (FACS). GTX628507
Anti-CD45RA antibody [GT0014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628507

Cat. No. GTX628507

Host

Mouse

Clonality

Monoclonal

Clone Name

GT0014

Isotype

IgG1

Application

WB, ICC/IF, FACS

Reactivity

Human, Rabbit
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS 1:1:50-1:200
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Positive Control

Raji , Raji membrane extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human CD45RA

Purification

Affinity purified by Protein G.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888043

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD45RA antibody [GT0014] used in Flow cytometry (FACS). GTX628507

GTX628507 FACS Image

CD45RA antibody [GT0014] (GTX628507) detects CD45RA protein by flow cytometry analysis.
Sample: mouse splenocytes cell.
Left: Unlabelled sample was used as a control.
Right: CD45RA antibody [GT0014] (GTX628507) dilution: 1:50.
Acquisition of 40,000 events were collected for FACS analysis.

Anti-CD45RA antibody [GT0014] used in Western Blot (WB). GTX628507

GTX628507 WB Image

Raji whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45RA antibody [GT0014] (GTX628507) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 24405902

Anti-CD45RA antibody [GT0014] used in Flow cytometry (FACS). GTX628507

GTX628507 FACS Image

CD45RA antibody [GT0014] (GTX628507) detects CD45RA protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD45RA antibody [GT0014] (GTX628507) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-CD45RA antibody [GT0014] used in Flow cytometry (FACS). GTX628507

GTX628507 FACS Image

CD45RA antibody [GT0014] (GTX628507) detects CD45RA protein by flow cytometry analysis.
Sample: THP-1 cell fixed in 4% paraformaldehyde at 4oC for 15 min.
Black: Isotype control dilution: 1:100.
Green: CD45 antibody [GT0014] (GTX628507) dilution: 1:100.

Anti-CD45RA antibody [GT0014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628507

GTX628507 ICC/IF Image

CD45RA antibody [GT0014] (GTX628507) detects CD45RA protein at cell membrane by immunofluorescent analysis.
Sample: Raji cells were fixed in ice-cold MeOH for 5 min.
Green: CD45RA protein stained by CD45RA antibody [GT0014] (GTX628507) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations