Glypican 1 antibody [N3C3]

Name: Glypican 1 antibody [N3C3]
Brand: GeneTex
SKU: GTX104557
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

Anti-Glypican 1 antibody [N3C3] used in Flow cytometry (FACS). GTX104557

Anti-Glypican 1 antibody [N3C3] used in Immunoprecipitation (IP). GTX104557

Anti-Glypican 1 antibody [N3C3] used in ELISA (ELISA). GTX104557

Anti-Glypican 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104557

Anti-Glypican 1 antibody [N3C3] used in Western Blot and Immunoprecipitation (WB IP). GTX104557

Cat. No. GTX104557

Reference 11

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, IP, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Glypican 1 products

View product citations for antibody GTX104557 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
FACS	1:50-1:200
IP	1:100-1:500
ELISA	1:1000-1:10000

Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

MDA-MB-231 , MDA-MB-231 (24 μg/ml Tunicamycin for 16hr) , U87-MG membrane extracts , U87-MG conditioned medium , Mouse brain , mouse fetal brain , A431 , MCF-7 , AsPC-1

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950436

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glypican 1 , glypican

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]

Database

Gene ID: 2817 GPC1
UniProt: P35052 GPC1

Research Area

DATA IMAGES

GTX104557 IHC-P Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).
Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104557 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104557 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX104557 WB Image

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

GTX104557 FACS Image

Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis.
Sample: A431 cell.
Black: Unlabelled sample was used as a control.
Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:100.
Acquisition of 20,000 events were collected for FACS analysis.

GTX104557 IP Image

Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 μg of Glypican 1 antibody [N3C3] (GTX104557).
Western blot analysis was performed using Glypican 1 antibody [N3C3] (GTX104557).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104557 ELISA Image

An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (GTX104557) at concentration ranged from 7.5 to 240 ng/mL.

GTX104557 WB Image

U87-MG whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

GTX104557 WB Image

Mouse tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104557 IHC-P Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104557 ICC/IF Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX104557 WB Image

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

GTX104557 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

GTX104557 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

GTX104557 WB Image

A431 whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104557 WB IP Image

The data was published in the journal Nat Commun in 2017. PMID: 28102194

GTX104557 IHC-P Image

The data was published in the journal Nat Commun in 2017. PMID: 28102194

GTX104557 WB IP Image

The data was published in the journal Nat Commun in 2017. PMID: 28102194

GTX104557 IHC-P Image

The data was published in the journal Nat Commun in 2017. PMID: 28102194

REFERENCE

Application Reference

S Uchida et al. Neoplasia 2024; 50 : 100982 Glypican-1-targeted antibody–drug conjugate inhibits the growth of glypican-1-positive glioblastoma

Sukhneeraj P Kaur et al. Sci Rep 2021; 11 (1) : 9262 Inhibition of glypican-1 expression induces an activated fibroblast phenotype in a human bone marrow-derived stromal cell-line.

Application : WB

Reactivity : Human

Munekage E et al. Neoplasia 2021; 23 (9) : 939-950 A glypican-1-targeted antibody-drug conjugate exhibits potent tumor?growth inhibition in glypican-1-positive pancreatic cancer and esophageal squamous cell carcinoma.

Application : IHC-P

Reactivity : Human

Zhang J et al. Ecotoxicol Environ Saf 2021; 218 : 112257 Increased secretion of VEGF-C from SiO₂-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.

Application : WB, IHC-P

Reactivity : Mouse

Emmanouilidi A et al. Proteomics 2019; 19 (8) : e1800158 Oncogenic and Non-Malignant Pancreatic Exosome Cargo Reveal Distinct Expression of Oncogenic and Prognostic Factors Involved in Tumor Invasion and Metastasis.

Application : WB

Reactivity : Human

Show More +

Submit a Reference

REVIEW