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PBX1 antibody

Orthogonal
Anti-PBX1 antibody used in Immunoprecipitation (IP). GTX113242
Anti-PBX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113242
Anti-PBX1 antibody used in Western Blot (WB). GTX113242

Cat. No. GTX113242

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PBX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PBX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169359

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PBX homeobox 1 , CAKUHED

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-PBX1 antibody used in Immunoprecipitation (IP). GTX113242

GTX113242 IP Image

PBX1 antibody immunoprecipitates PBX1 protein in IP experiments.
IP Sample: 293T whole cell lysate/extract
A. Control with 2 μg of preimmune rabbit IgG
B. Immunoprecipitation of PBX1 protein by 2 μg of PBX1 antibody (GTX113242)
12 % SDS-PAGE
The immunoprecipitated PBX1 protein was detected by PBX1 antibody (GTX113242) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PBX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113242

GTX113242 ICC/IF Image

PBX1 antibody detects PBX1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PBX1 stained by PBX1 antibody (GTX113242) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-PBX1 antibody used in Western Blot (WB). GTX113242

GTX113242 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PBX1 antibody (GTX113242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Dutta S et al. Res Sq 2023; Understanding the role of Pax5 in development of taxane-resistant neuroendocrine like prostate cancers.
S Dutta et al. Research Square 2023; Understanding the role of Pax5 in development of taxane-resistant neuroendocrine like prostate cancers
Fang CH et al. J Biomed Sci 2020; 27 (1) : 42 A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and 帣-catenin-ABCG2 signaling.
Application : WB
Reactivity : Human
Tang W et al. Onco Targets Ther 2018; 11 : 2319-2332 MicroRNA-150 suppresses triple-negative breast cancer metastasis through targeting HMGA2.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169