GeneTex
United States (US)

14-3-3 beta + epsilon + zeta antibody [3C8]

14-3-3-beta-epsilon-zeta-antibody-3C8_WB_GTX12346-1_18121409_468.jpg

Cat No. GTX12346

Host Mouse
Clonality Monoclonal
Clone Name 3C8
Isotype IgG2b
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

WB: Use at a concentration of 1 - 10 μg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa). Optimal dilutions/concentrations should be determined by the end user.

Positive Control

HeLa Cell Lysate, Mouse Brain Tissue Extract, Rat Brain Tissue Extract

Specificity/Sensitivity

This antibody detects an ~30 kDa protein, corresponding to the apparent molecular mass of 14-3-3 beta, 14-3-3 epsilon or 14-3-3 zeta on SDS-PAGE immunoblots, in samples from human, mouse and rat origins.
PROPERTIES

Form

Liquid

Buffer

Preservative: None Constituents: 50% Glycerol, PBS. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human 14-3-3 beta fusion protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

1433 beta + epsilon + zeta, 1433, GW128, 14-3-3E, 14-3-3-zeta, HS1, FLJ45465, KCIP-1, FLJ53559, MGC111427, YWHAA, MGC126532, MDCR, MGC138156, MDS, YWHAD, KCIP 1, 14 3 3 zeta, KCIP1, 1433zeta

Cellular Localization

Cytoplasmic

Background

14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

Research Area

DATA IMAGES
14-3-3-beta-epsilon-zeta-antibody-3C8_WB_GTX12346-1_18121409_468.jpg

GTX12346 WB Image

Western blot analysis probed with GTX12346
Lane 1: Human HeLa whole extract
Lane 2: Mouse brain extract
Lane 3: Rat brain extract

REFERENCE
Package List Price ($)
$ 319