GeneTex
United States (US)

14-3-3 eta antibody

GTX104758_39666_20141128_IHC_19011506_849.jpg
GTX104758_WB_19011506_226.jpg
GTX104758_39666_IFA_19011506_937.jpg

Cat No. GTX104758

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 eta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Eta,Ywha1,Ywhah

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia. [provided by RefSeq]

Database

DATA IMAGES
GTX104758_39666_20141128_IHC_19011506_849.jpg

GTX104758 IHC-P Image

14-3-3 eta antibody detects 14-3-3 eta protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
14-3-3 eta antibody (GTX104758) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104758_WB_19011506_226.jpg

GTX104758 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 12% SDS PAGE
GTX104758 diluted at 1:1000

GTX104758_39666_IFA_19011506_937.jpg

GTX104758 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 eta(GTX104758) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169