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5-HT2B receptor antibody

Orthogonal
Anti-5-HT2B receptor antibody used in Western Blot (WB). GTX129900
Anti-5-HT2B receptor antibody used in Western Blot (WB). GTX129900

Cat. No. GTX129900

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all 5-HT2B receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

mouse liver , SH-SY5Y membrane extract

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human 5-HT2B receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886117

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5-hydroxytryptamine receptor 2B , 5-HT(2B) , 5-HT-2B , 5-HT2B

Cellular Localization

Cell membrane

Background

Multiple receptor subtypes of serotonin neurotransmitters with multiple physiologic functions have been recognized. The 5-HT-2 receptors mediate many of the central and peripheral physiologic functions of serotonin. Cardiovascular effects include contraction of blood vessels and shape changes in platelets; central nervous system effects include neuronal sensitization to tactile stimuli and mediation of hallucinogenic effects of phenylisopropylamine hallucinogens.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-5-HT2B receptor antibody used in Western Blot (WB). GTX129900

GTX129900 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2B Receptor antibody (GTX129900) diluted at 1:1000.

Anti-5-HT2B receptor antibody used in Western Blot (WB). GTX129900

GTX129900 WB Image

Orthogonal

Boiled and unboiled SH-SY5Y cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2B Receptor antibody (GTX129900) diluted at 1:1000. (WCE: whole cell extract; ME: membrane extract)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169