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ABCG1 antibody

Orthogonal
Anti-ABCG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30598
Anti-ABCG1 antibody used in Western Blot (WB). GTX30598
Anti-ABCG1 antibody used in Western Blot (WB). GTX30598

Cat. No. GTX30598

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, in vitro

Reactivity

 Human, Mouse, Rat, Rabbit, Hamster, Monkey
Package
100 μl ($449)
See all ABCG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P 1:100 - 1:400
in vitro Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_423876

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily G member 1 , ABC8 , WHITE1

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ABCG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30598

GTX30598 IHC-P Image

IHC-P analysis of human lung tissue using GTX30598 ABCG1 antibody.

Anti-ABCG1 antibody used in Western Blot (WB). GTX30598

GTX30598 WB Image

WB analysis of mouse peritoneal macrophages using GTX30598 ABCG1 antibody.
Lane 1 : untreated mouse peritoneal macrophages (positive control)
Lane 2 : 22-(R)-hydrocholesterol treated macrophages
Lane 3 : T0901713 treated macrophages
Lane 4 : T0901713 treated macrophages

Anti-ABCG1 antibody used in Western Blot (WB). GTX30598

GTX30598 WB Image

Orthogonal

WB analysis of RAW264.7 (A), and RAW264.7 treated with 10uM T09 18 hr (B) cell lysate using GTX30598 ABCG1 antibody.
Dilution : 2 μg/ml

REFERENCE

Application Reference

Wang G et al. Cell Death Dis 2021; 12 (3) : 254 CTRP12 ameliorates atherosclerosis by promoting cholesterol efflux and inhibiting inflammatory response via the miR-155-5p/LXRα pathway.
Application : WB
Reactivity : Human
Sabeva NS et al. J Nutr Biochem 2011; 22 (8) : 777-83 Phytosterols differentially influence ABC transporter expression, cholesterol efflux and inflammatory cytokine secretion in macrophage foam cells.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449