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ABCG5 antibody [1B5E10]

Anti-ABCG5 antibody [1B5E10] used in Flow cytometry (FACS). GTX60788
Anti-ABCG5 antibody [1B5E10] used in IHC (Paraffin sections) (IHC-P). GTX60788
Anti-ABCG5 antibody [1B5E10] used in Western Blot (WB). GTX60788
Anti-ABCG5 antibody [1B5E10] used in Western Blot (WB). GTX60788
Anti-ABCG5 antibody [1B5E10] used in IHC (Paraffin sections) (IHC-P). GTX60788
Anti-ABCG5 antibody [1B5E10] used in ELISA (ELISA). GTX60788

Cat. No. GTX60788

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B5E10

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all ABCG5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ABCG5 (AA: 306-367) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily G member 5 , STSL

Cellular Localization

Cell membrane,Apical cell membrane

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ABCG5 antibody [1B5E10] used in Flow cytometry (FACS). GTX60788

GTX60788 FACS Image

FACS analysis of A549 cells using GTX60788 ABCG5 antibody [1B5E10].
Green : ABCG5
Red : negative control

Anti-ABCG5 antibody [1B5E10] used in IHC (Paraffin sections) (IHC-P). GTX60788

GTX60788 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60788 ABCG5 antibody [1B5E10].

Anti-ABCG5 antibody [1B5E10] used in Western Blot (WB). GTX60788

GTX60788 WB Image

WB analysis of HEK293 (1) and ABCG5 (AA: 306-367)-hIgGFc transfected HEK293 (2) cell lysate using GTX60788 ABCG5 antibody [1B5E10].

Anti-ABCG5 antibody [1B5E10] used in Western Blot (WB). GTX60788

GTX60788 WB Image

WB analysis of HL7702 (1), RAJI (2) and Jurkat (3) cell lysate using GTX60788 ABCG5 antibody [1B5E10].

Anti-ABCG5 antibody [1B5E10] used in IHC (Paraffin sections) (IHC-P). GTX60788

GTX60788 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60788 ABCG5 antibody [1B5E10].

Anti-ABCG5 antibody [1B5E10] used in ELISA (ELISA). GTX60788

GTX60788 ELISA Image

ELISA analysis of antigen using GTX60788 ABCG5 antibody [1B5E10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

Application Reference

Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
Application : WB
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409