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ABCG8 antibody

Anti-ABCG8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65624
Anti-ABCG8 antibody used in Western Blot (WB). GTX65624

Cat. No. GTX65624

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all ABCG8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ABCG8 (NP_071882.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily G member 8 , GBD4 , STSL

Cellular Localization

Cell membrane,Apical cell membrane

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ABCG8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65624

GTX65624 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX65624 ABCG8 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ABCG8 antibody used in Western Blot (WB). GTX65624

GTX65624 WB Image

WB analysis of LO2 cell lysate using GTX65624 ABCG8 antibody.
Dilution : 1:1000
Loading : 25μg per lane
Detection: ECL Enhanced Kit

REFERENCE

Application Reference

Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
Application : WB
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399