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ACAA2 antibody

Anti-ACAA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115417
Anti-ACAA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115417
Anti-ACAA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115417
Anti-ACAA2 antibody used in Western Blot (WB). GTX115417

Cat. No. GTX115417

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ACAA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACAA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885361

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acetyl-CoA acyltransferase 2 , DSAEC

Cellular Localization

Mitochondrion

Background

The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACAA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115417

GTX115417 IHC-P Image

ACAA2 antibody detects ACAA2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ACAA2 antibody (GTX115417) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACAA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115417

GTX115417 IHC-P Image

ACAA2 antibody detects ACAA2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ACAA2 antibody (GTX115417) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACAA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115417

GTX115417 ICC/IF Image

ACAA2 antibody detects ACAA2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACAA2 protein stained by ACAA2 antibody (GTX115417) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ACAA2 antibody used in Western Blot (WB). GTX115417

GTX115417 WB Image

ACAA2 antibody detects ACAA2 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
ACAA2 antibody (GTX115417) dilution: 1:1000

REFERENCE

Application Reference

Marco Rosina et al. Cell Metab 2022; 34 (4) : 533-548.e12 Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.
Application : WB
Reactivity : Mouse
Wang W et al. Biochem Biophys Res Commun 2021; 576 : 15-21 ACAA2 is a ligand-dependent coactivator for thyroid hormone receptor β1.
Application : WB
Reactivity : Mouse
Wei Zhong Zhu et al. Proteome Sci 2020; 18 (1) : 11 Sex impacts cardiac function and the proteome response to thyroid hormone in aged mice.
Application : WB
Reactivity : Mouse
Wei Zhong Zhu et al. Proteome Science 2020; (Epub) Sex Impacts Cardiac Function and the Proteome Response to Thyroid Hormone in Aged Mice.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169