GeneTex

  • Country / Location Selection

United States (US)

ADAMTS5 antibody

Anti-ADAMTS5 antibody used in Western Blot (WB). GTX100332
Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332
Anti-ADAMTS5 antibody used in Western Blot (WB). GTX100332
Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332
Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332
Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332
Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332
Anti-ADAMTS5 antibody used in Western Blot (WB). GTX100332
Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332

Cat. No. GTX100332

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ADAMTS5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

HCT116 , MCF-7 , JC

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ADAMTS5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240417

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADAM metallopeptidase with thrombospondin type 1 motif 5 , ADAM-TS 11 , ADAM-TS 5 , ADAM-TS5 , ADAMTS-11 , ADAMTS-5 , ADAMTS11 , ADMP-2

Background

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADAMTS5 antibody used in Western Blot (WB). GTX100332

GTX100332 WB Image

Sample (30 ug of whole cell lysate)
A: JC
7.5% SDS PAGE
GTX100332 diluted at 1:1000

Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332

GTX100332 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ADAMTS5(GTX100332) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADAMTS5 antibody used in Western Blot (WB). GTX100332

GTX100332 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
B: MCF-7
7.5% SDS PAGE
GTX100332 diluted at 1:1000

Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332

GTX100332 IHC-P Image

The data was published in the 2020 in Nat Commun. PMID: 32859940

Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332

GTX100332 IHC-P Image

The data was published in the journal Nat Commun in 2019. PMID: 31160553

Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332

GTX100332 IHC-P Image

The data was published in the journal Nat Commun in 2019. PMID: 31160553

Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332

GTX100332 IHC-P Image

The data was published in the journal Nat Commun in 2019. PMID: 31160553

Anti-ADAMTS5 antibody used in Western Blot (WB). GTX100332

GTX100332 WB Image

The data was published in the journal Dis Markers in 2019. PMID: 31929842

Anti-ADAMTS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX100332

GTX100332 IHC-P Image

The data was published in the journal Dis Markers in 2019. PMID: 31929842

REFERENCE

Application Reference

Kim J et al. Nat Commun 2024; 15 (1) : 1024 5-aminosalicylic acid suppresses osteoarthritis through the OSCAR-PPARγ axis.
A Nekomoto et al. Sci Rep 2023; 13 (1) : 15354 Feasibility of administration of calcitonin gene-related peptide receptor antagonist on attenuation of pain and progression in osteoarthritis
Reactivity : Mouse
Hsueh YH et al. J Orthop Translat 2023; 38 : 141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Sanada Y et al. Arthritis Res Ther 2022; 24 (1) : 235 Senescence-accelerated mice prone 8 (SAMP8) in male as a spontaneous osteoarthritis model.
Application : IHC-P
Reactivity : Mouse
Fujiwara Y et al. Front Cell Dev Biol 2022; 10 : 1043259 miR-23a/b clusters are not essential for the pathogenesis of osteoarthritis in mouse aging and post-traumatic models.
Application : IHC-P
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

There are currently no reviews for ADAMTS5 antibody (GTX100332). Be the first to share your experience with this product.
Submit a Review
Application Reference
Kim J et al. Nat Commun 2024; 15 (1):1024 5-aminosalicylic acid suppresses osteoarthritis through the OSCAR-PPARγ axis.
A Nekomoto et al. Sci Rep 2023; 13 (1):15354 Feasibility of administration of calcitonin gene-related peptide receptor antagonist on attenuation of pain and progression in osteoarthritis
Reactivity : Mouse
Hsueh YH et al. J Orthop Translat 2023; 38:141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Sanada Y et al. Arthritis Res Ther 2022; 24 (1):235 Senescence-accelerated mice prone 8 (SAMP8) in male as a spontaneous osteoarthritis model.
Application : IHC-P
Reactivity : Mouse
Fujiwara Y et al. Front Cell Dev Biol 2022; 10:1043259 miR-23a/b clusters are not essential for the pathogenesis of osteoarthritis in mouse aging and post-traumatic models.
Application : IHC-P
Reactivity : Mouse
Park DR et al. Nat Commun 2020; 11 (1):4343 Osteoclast-associated receptor blockade prevents articular cartilage destruction via chondrocyte apoptosis regulation.
Application : IHC-P
Reactivity : Human
Kanemitsu M et al. J Orthop Sci 2020; (Epub) Role of vasoactive intestinal peptide in the progression of osteoarthritis through bone sclerosis and angiogenesis in subchondral bone.
Wang Z et al. Dis Markers 2019; 2019:6129748 ADAMTS-5 Decreases in Coronary Arteries and Plasma from Patients with Coronary Artery Disease.
Mokuda S et al. Nat Commun 2019; 10 (1):2429 Wwp2 maintains cartilage homeostasis through regulation of Adamts5.
Liu S et al. PLoS One 2015; 10 (6):e0129999 Connexin43 Mediated Delivery of ADAMTS5 Targeting siRNAs from Mesenchymal Stem Cells to Synovial Fibroblasts.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169