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ADFP antibody

Anti-ADFP antibody used in Western Blot (WB). GTX02576
Anti-ADFP antibody used in Western Blot (WB). GTX02576
Anti-ADFP antibody used in IHC (Paraffin sections) and In situ hybridization (IHC-P ISH). GTX02576
Anti-ADFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02576
Anti-ADFP antibody used in IHC (Paraffin sections) (IHC-P). GTX02576
Anti-ADFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02576

Cat. No. GTX02576

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ISH

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ADFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 1:50-1:200
IHC-P 1:200
ISH Assay dependent

Note :

IHC-P
Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide made to a C-terminal region of mouse ADFP (within residues 350-425) [Swiss-Prot# P43883].

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

perilipin 2 , AA407157 , ADPH , Adfp , Adrp

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-ADFP antibody used in Western Blot (WB). GTX02576

GTX02576 WB Image

WB analysis of HepG2 lysate uisng GTX02576 ADFP Antibody.
Dilution : 0.5 mg/ml

Anti-ADFP antibody used in Western Blot (WB). GTX02576

GTX02576 WB Image

WB analysis of mouse liver lysate using GTX02576 ADFP Antibody.

Anti-ADFP antibody used in IHC (Paraffin sections) and In situ hybridization (IHC-P ISH). GTX02576

GTX02576 IHC-P ISH Image

IHC-P/ISH analysis of mouse intestine tissue using GTX02576 ADFP Antibody. Specific staining was localized to intestinal villi.
Dilution : 1:150

Anti-ADFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02576

GTX02576 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX02576 ADFP Antibody.
Green : Primary antibody
Blue : Nuclei were counterstained using Hoechst 33258
Dilution : 1:50

Anti-ADFP antibody used in IHC (Paraffin sections) (IHC-P). GTX02576

GTX02576 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX02576 ADFP Antibody.

Anti-ADFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02576

GTX02576 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HepG2 cells using GTX02576 ADFP Antibody.
Green : Primary antibody
Red : Alpha tubulin (DM1A)
Blue : Nuclei were counterstained with DAPI
Permeabilization : 1X TBS + 0.5% Triton-X100
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399