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AES antibody

Anti-AES antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16982
Anti-AES antibody used in Western Blot (WB). GTX16982
Anti-AES antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16982

Cat. No. GTX16982

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all AES products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 4 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

HeLa Cell Lysate , HeLa Cell Slide(GTX25529) , HEK293 Cell Lysate(GTX27902)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES.The immunogen is located within the last 50 amino acids of AES.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TLE family member 5, transcriptional modulator , AES , AES-1 , AES-2 , ESP1 , GRG , GRG5 , Grg-5

Cellular Localization

Nucleus

Background

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AES antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16982

GTX16982 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX16982 AES antibody.
Working concentration : 20 μg/ml

Anti-AES antibody used in Western Blot (WB). GTX16982

GTX16982 WB Image

WB analysis of 293 cell lysate using GTX16982 AES antibody.
Working concentration : (A) 1, (B) 2, and (C) 4 μg/ml

Anti-AES antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16982

GTX16982 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX16982 AES antibody.
Working concentration : 10 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399