APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
2 μg/mL |
4 μg/mL |
1:10-1:100 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to residues Q(31) F R Q R K A Q S D G Q S P S(45) of human AKAP450.
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_11174025
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
A-kinase anchoring protein 9 , AKAP-9 , AKAP350 , AKAP450 , CG-NAP , HYPERION , LQT11 , MU-RMS-40.16A , PPP1R45 , PRKA9 , YOTIAO
Cellular Localization
Golgi apparatus,Cytoplasm
Background
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq, Aug 2008]
Database
Research Area
DATA IMAGES
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GTX79473 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX79473 AKAP9 antibody [17G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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GTX79473 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX79473 AKAP9 antibody [17G10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 ºC
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GTX79473 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX79473 AKAP9 antibody [17G10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC
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GTX79473 ICC/IF Image
ICC/IF analysis of A431 cells using GTX79473 AKAP9 antibody [17G10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 ºC
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REFERENCE
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REVIEW
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