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AKAP9 antibody, C-term

Anti-AKAP9 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89583
Anti-AKAP9 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89583

Cat No. GTX89583

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF 10μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells.

Not tested in other applications.

Calculated MW

454 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89583-PEP).

Specificity/Sensitivity

This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SGSTTQFHAGMR, from the C Terminus of the protein sequence according to NP_005742.4; NP_671714.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

A-Kinase Anchoring Protein 9,Akap-9,Akap350,Akap450,Cg-Nap,Hyperion,Lqt11,Mu-Rms-40.16A,Ppp1R45,Prka9,Yotiao,Akap9

Cellular Localization

Cytoplasmic in parietal cells.

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES
Anti-AKAP9 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89583

GTX89583 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX89583 AKAP9 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

Anti-AKAP9 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89583

GTX89583 ICC/IF Image

ICC/IF analysis of PFA fixed A431 cells using GTX89583 AKAP9 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

REFERENCE
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Package List Price ($)
$ 329