GeneTex
  • Country / Location Selection

United States (US)

AKT (phospho Ser473) antibody [104A282]

Anti-AKT (phospho Ser473) antibody [104A282] used in Western Blot (WB). GTX11901
Anti-AKT (phospho Ser473) antibody [104A282] used in Western Blot (WB). GTX11901
Anti-AKT (phospho Ser473) antibody [104A282] used in IHC (Paraffin sections) (IHC-P). GTX11901
Anti-AKT (phospho Ser473) antibody [104A282] used in IHC (Paraffin sections) (IHC-P). GTX11901

Cat No. GTX11901

Host Mouse
Clonality Monoclonal
Clone Name 104A282
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Zebrafish, Chicken
Package
50 μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:200 - 1:250
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Xenopus(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-AKT (phospho Ser473) antibody [104A282] used in Western Blot (WB). GTX11901

GTX11901 WB Image

WB analysis of 3T3 cells treated with and without PDGF (50 ng/ml) using GTX11901 AKT (phospho Ser473) antibody [104A282] and AKT1 antibody.
Dilution : 2 μg/ml

Anti-AKT (phospho Ser473) antibody [104A282] used in Western Blot (WB). GTX11901

GTX11901 WB Image

WB analysis of untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate using GTX11901 AKT (phospho Ser473) antibody [104A282].
Dilution : 2 μg/ml

Anti-AKT (phospho Ser473) antibody [104A282] used in IHC (Paraffin sections) (IHC-P). GTX11901

GTX11901 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX11901 AKT (phospho Ser473) antibody [104A282].
Dilution : 1:250
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

Anti-AKT (phospho Ser473) antibody [104A282] used in IHC (Paraffin sections) (IHC-P). GTX11901

GTX11901 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX11901 AKT (phospho Ser473) antibody [104A282].
Dilution : 1:250
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

REFERENCE
REVIEW
There are currently no reviews for AKT (phospho Ser473) antibody [104A282] (GTX11901). Be the first to share your experience with this product.
Package List Price ($)
$ 319