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AKT (phospho Ser473) antibody [104A282]

Orthogonal
Anti-AKT (phospho Ser473) antibody [104A282] used in Western Blot (WB). GTX11901
Anti-AKT (phospho Ser473) antibody [104A282] used in IHC (Paraffin sections) (IHC-P). GTX11901
Anti-AKT (phospho Ser473) antibody [104A282] used in IHC (Paraffin sections) (IHC-P). GTX11901
Anti-AKT (phospho Ser473) antibody [104A282] used in Western Blot (WB). GTX11901

Cat. No. GTX11901

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 104A282

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Zebrafish, Chicken
Package
50 μg ($399)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:200 - 1:250
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372736

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKT (phospho Ser473) antibody [104A282] used in Western Blot (WB). GTX11901

GTX11901 WB Image

Orthogonal

WB analysis of 3T3 cells treated with and without PDGF (50 ng/ml) using GTX11901 AKT (phospho Ser473) antibody [104A282] and AKT1 antibody.
Dilution : 2 μg/ml

Anti-AKT (phospho Ser473) antibody [104A282] used in IHC (Paraffin sections) (IHC-P). GTX11901

GTX11901 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX11901 AKT (phospho Ser473) antibody [104A282].
Dilution : 1:250
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

Anti-AKT (phospho Ser473) antibody [104A282] used in IHC (Paraffin sections) (IHC-P). GTX11901

GTX11901 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX11901 AKT (phospho Ser473) antibody [104A282].
Dilution : 1:250
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

Anti-AKT (phospho Ser473) antibody [104A282] used in Western Blot (WB). GTX11901

GTX11901 WB Image

Orthogonal

WB analysis of untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate using GTX11901 AKT (phospho Ser473) antibody [104A282].
Dilution : 2 μg/ml

REFERENCE

Application Reference

Shangguan H et al. G3 (Bethesda) 2024; 14 (3) Knockdown of Kmt2d leads to growth impairment by activating the Akt/β-catenin signaling pathway.
Hung-Pin Chang et al. Life (Basel) 2022; 12 (5) High Mobility Group A 1 Expression as a Poor Prognostic Marker Associated with Tumor Invasiveness in Gastric Cancer.
Application : IHC-P
Reactivity : Human
Chen YJ et al. Gynecol Oncol 2013; 131 (3) : 734-43 The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.
Application : WB
Reactivity : Human
Matsuda N et al. Cardiovasc Res 2007; 76 (1) : 132-40 Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock.
Application : WB
Reactivity : Mouse

Clone Reference

Luty WH et al. J Immunol 2007; 179 (2):819-29 Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399