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AKT (phospho Ser473) antibody

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50128
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50128

Cat No. GTX50128

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Monkey
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from human AKT.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKT serine/threonine kinase 1,AKT serine/threonine kinase 2,AKT serine/threonine kinase 3,AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-PK-gamma,RAC-gamma,STK-2

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Membrane

Database

Research Area

DATA IMAGES
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128

GTX50128 WB Image

WB analysis of extracts from 293 cells treated with EGF or calf intestinal phosphatase (CIP) using GTX50128 AKT (phospho Ser473) antibody.

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50128

GTX50128 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128

GTX50128 WB Image

WB analysis of extracts from SK-BR-3 cells untreated, treated with insulin + EGF, U0126, or LY294002 using GTX50128 AKT (phospho Ser473) antibody.

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50128

GTX50128 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE
REVIEW
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Package List Price ($)
$ 319