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AKT (phospho Ser473) antibody

Orthogonal
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50128
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50128
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128

Cat. No. GTX50128

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from human AKT.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11170645

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT serine/threonine kinase 2 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Membrane

Database

Research Area

DATA IMAGES

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50128

GTX50128 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50128

GTX50128 IHC-P Image

Orthogonal

IHC-P analysis of human lung carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128

GTX50128 WB Image

Orthogonal

WB analysis of extracts from 293 cells treated with EGF or calf intestinal phosphatase (CIP) using GTX50128 AKT (phospho Ser473) antibody.

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128

GTX50128 WB Image

Orthogonal

WB analysis of extracts from SK-BR-3 cells untreated, treated with insulin + EGF, U0126, or LY294002 using GTX50128 AKT (phospho Ser473) antibody.

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50128

GTX50128 WB Image

Orthogonal

The data was published in the 2019 in Aging (Albany NY). PMID: 31584878

REFERENCE

Application Reference

Lopez-Toledo G et al. Front Aging Neurosci 2022; 14 : 921573 Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.
Application : WB
Reactivity : Human
Zhang-Hua Chen et al. Genome Med 2021; 13 (1) : 146 The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma.
Application : WB
Reactivity : Human
Pan CH et al. Chem Biol Interact 2020; 332 : 109304
Application : WB
Reactivity : Human
Pan CH et al. Aging (Albany NY) 2019; 11 (19) : 8085-8102 Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta.
Application : WB
Reactivity : Rat
Cheng YM et al. Oncotarget 2018; 9 (41) : 26342-26352 PSMD4 is a novel therapeutic target in chemoresistant colorectal cancer activated by cytoplasmic localization of Nrf2.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Lopez-Toledo G et al. Front Aging Neurosci 2022; 14:921573 Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.
Application : WB
Reactivity : Human
Zhang-Hua Chen et al. Genome Med 2021; 13 (1):146 The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma.
Application : WB
Reactivity : Human
Pan CH et al. Chem Biol Interact 2020; 332:109304
Application : WB
Reactivity : Human
Pan CH et al. Aging (Albany NY) 2019; 11 (19):8085-8102 Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta.
Application : WB
Reactivity : Rat
Cheng YM et al. Oncotarget 2018; 9 (41):26342-26352 PSMD4 is a novel therapeutic target in chemoresistant colorectal cancer activated by cytoplasmic localization of Nrf2.
Application : WB
Reactivity : Human
Zhao J et al. J Steroid Biochem Mol Biol 2018; (Epub) Orchiectomy and * differentially regulate synaptic plasticity and spatial memory in a manner that is mediated by SRC-1 in the hippocampus of male mice.
Application : WB
Reactivity : Mouse
Lin PL et al. Free Radic Biol Med 2016; 95:121-132 Cytoplasmic localization of Nrf2 promotes colorectal cancer with more aggressive tumors via upregulation of PSMD4.
Application : WB, IHC-P
Reactivity : Monkey
Chun-Hao Tsai et al. Toxicology Research 2016; 5:151-159 Identification of Id1 as a downstream effector for arsenic-promoted angiogenesis via PI3K/Akt, NF-κB and NOS signaling.
Application : WB
Reactivity : Human, Mouse
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399