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AKT (phospho Ser473) antibody

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50186
Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50186
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50186

Cat. No. GTX50186

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Product Note

This antibody is raised against human AKT2 phosphorylated at Ser474. Based on sequence homology, it is predicted to react with AKT1 and AKT3 when phosphorylated at the corresponding residues - AKT1 Ser473 and AKT3 Ser472.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from human AKT.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT serine/threonine kinase 2 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Membrane

Database

Research Area

DATA IMAGES

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50186

GTX50186 WB Image

WB analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GTX50186 AKT (phospho Ser473) antibody.

Anti-AKT (phospho Ser473) antibody used in IHC (Paraffin sections) (IHC-P). GTX50186

GTX50186 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50186 AKT (phospho Ser473) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX50186

GTX50186 WB Image

WB analysis of extracts from 293 cells treated with serum or calf intestinal phosphatase (CIP) using GTX50186 AKT (phospho Ser473) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399