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AKT (phospho Ser473) antibody

Orthogonal
Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX59559

Cat. No. GTX59559

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-4 μg/ml
IP 10-20 μg/ml
Not tested in other applications.

Product Note

The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol, 1% BSA

Preservative

0.02% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10720029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thymoma viral proto-oncogene 1 , thymoma viral proto-oncogene 2 , thymoma viral proto-oncogene 3 , Akt , LTR-akt , PKB , PKB/Akt , PKBalpha , Rac , 2410016A19Rik , AW554154 , PKB , PKBbeta , AI851531 , D930002M15Rik , Nmf350

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Database

Research Area

DATA IMAGES

Anti-AKT (phospho Ser473) antibody used in Western Blot (WB). GTX59559

GTX59559 WB Image

Orthogonal

WB analysis of NIH3T3 cells treated without (left lane) or with (right lane) PDGF lysate using GTX59559 AKT (phospho Ser473) antibody.

REFERENCE

Application Reference

Lo WC et al. Cell Transplant 2021; 30 : 9636897211045319 Platelet-Derived Biomaterials Inhibit Nicotine-Induced Intervertebral Disc Degeneration Through Regulating IGF-1/AKT/IRS-1 Signaling Axis.
Application : WB
Reactivity : Human
Lo WC et al. Cell Transplant 2020; 29 : 9.6369E+14 Amelioration of Nicotine-Induced Osteoarthritis by Platelet-Derived Biomaterials Through Modulating IGF-1/AKT/IRS-1 Signaling Axis.
Application : WB
Reactivity : Human
Lu JW et al. Med Oncol 2015; 32 (7) : 206 mk-2206 induces apoptosis of aml cells and enhances the cytotoxicity of *.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399