AKT (phospho Thr308) antibody

Cat. No. GTX28933

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS, Dot, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μg ($589)

See all AKT1 products

View product citations for antibody GTX28933 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
IHC-P	1:200
FACS	Assay dependent
Dot	Assay dependent
ELISA	1:15000

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Product Note

This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phosphorylated synthetic peptide corresponding to residues surrounding threonine 308 of human AKT1 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_367526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Database

Gene ID: 207 AKT1
UniProt: P31749 AKT1

Research Area

DATA IMAGES

GTX28933 WB Image

Western Blot of Rabbit anti-Akt phospho T308 antibody (GTX28933). Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4ºC. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.

GTX28933 Dot Image

Dot blot analysis of various BSA-conjugated peptides using GTX28933 AKT (phospho Thr308) antibody.
Row A: AKT
Row B : AKT (phospho Thr308)
Row C and D : AKT (phospho Ser473)
Row E and F : CDC27 (phospho Thr244)
Row G : BSA
Lane 1 : 100 ng
Lane 2 : 33.33 ng
Lane 3 : 11.11 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1 μg/mL

GTX28933 ELISA Image

ELISA analysis of Non-phospho and phospho peptide using serially diluted GTX28933 AKT (phospho Thr308) antibody.
Blue : Phospho
Purple : Non-phospho
Coating : 0.1 μg

GTX28933 IHC-P Image

IHC-P analysis of human breast tissue using GTX28933 AKT (phospho Thr308) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

GTX28933 IHC-P Image

IHC-P analysis of human testis tissue using GTX28933 AKT (phospho Thr308) antibody.
Antigen retrieval : Heat mediated Leica Bond Epitope Retrieval Buffer 1 (Citrate solution, pH 6)

GTX28933 WB Image

WB analysis of various samples using GTX28933 AKT (phospho Thr308) antibody.
Lane 1 : Protein ladder
Lane 2 : Human spleen tissue lysate
Lane 3 : Human small intestine tissue lysate
Lane 4 : Human placenta tissue lysate
Lane 5 : Human skeletal muscle tissue lysate
Lane 6 : Human cerebellum tissue lysate
Lane 7 : Human lung tissue lysate
Lane 8 : Human tonsil tissue lysate
Lane 9 : Human thymus tissue lysate
Lane 10 : Protein ladder
Dilution : 1:1000

GTX28933 IHC-P Image

IHC-P analysis of human lung tissue using GTX28933 AKT (phospho Thr308) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

GTX28933 WB Image

WB analysis of GST-tagged AKT1 recombinant protein using GTX28933 AKT (phospho Thr308) antibody.
Lane 1 : In-active GST-tagged AKT1 recombinant protein
Lane 2 : Active GST-tagged AKT1 recombinant protein
Loading : 50 ng
Dilution : 1:1000

REFERENCE

Application Reference

Li H et al. Neurotox Res 2020; 37 (4) : 996-1008 Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway.

Application : WB

Reactivity : Rat

Liu J et al. Mol Med Rep 2019; 20 (1) : 33-40 miR?338?3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β?catenin signaling pathways.

Application : WB

Reactivity : Human

Piva MBR et al. Cancers (Basel) 2017; 9 (9) integrin activation contributes to lower * sensitivity in mv3 melanoma cells by inducing the wnt signalling pathway.

Application : FACS

Reactivity : Human

Chen CY et al. Oncotarget 2015; 6 (29) : 26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.

Application : WB

Reactivity : Human

Matsuda N et al. Cardiovasc Res 2007; 76 (1) : 132-40 Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock.

Application : WB

Reactivity : Mouse

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REVIEW

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Application Reference

Li H et al. Neurotox Res 2020; 37 (4):996-1008 Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway.

Application : WB

Reactivity : Rat

Liu J et al. Mol Med Rep 2019; 20 (1):33-40 miR?338?3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β?catenin signaling pathways.

Application : WB

Reactivity : Human

Piva MBR et al. Cancers (Basel) 2017; 9 (9) integrin activation contributes to lower * sensitivity in mv3 melanoma cells by inducing the wnt signalling pathway.

Application : FACS

Reactivity : Human

Chen CY et al. Oncotarget 2015; 6 (29):26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.

Application : WB

Reactivity : Human

Matsuda N et al. Cardiovasc Res 2007; 76 (1):132-40 Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock.

Application : WB

Reactivity : Mouse

SDS
Sodium Azide.pdf

Package	List Price ($)
100 μg	$ 589
Qty

AKT (phospho Thr308) antibody

Cat. No. GTX28933

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX28933 WB Image

GTX28933 Dot Image

GTX28933 ELISA Image

GTX28933 IHC-P Image

GTX28933 IHC-P Image

GTX28933 WB Image

GTX28933 IHC-P Image

GTX28933 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.