GeneTex
United States (US)

AKT (phospho Thr450) antibody

Anti-AKT (phospho Thr450) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50339
Anti-AKT (phospho Thr450) antibody used in Western Blot (WB). GTX50339
Anti-AKT (phospho Thr450) antibody used in IHC (Paraffin sections) (IHC-P). GTX50339

Cat No. GTX50339

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 450 (T-I-T(p)-P-P) derived from human AKT.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKT serine/threonine kinase 1,AKT serine/threonine kinase 2,AKT serine/threonine kinase 3,AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-PK-gamma,RAC-gamma,STK-2

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Membrane

Database

Research Area

DATA IMAGES
Anti-AKT (phospho Thr450) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50339

GTX50339 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50339 AKT (phospho Thr450) antibody.

Anti-AKT (phospho Thr450) antibody used in Western Blot (WB). GTX50339

GTX50339 WB Image

WB analysis of extracts from 293 cells untreated or treated with EGF using GTX50339 AKT (phospho Thr450) antibody.

Anti-AKT (phospho Thr450) antibody used in IHC (Paraffin sections) (IHC-P). GTX50339

GTX50339 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50339 AKT (phospho Thr450) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Package List Price ($)
$ 319