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AKT (phospho Tyr315) antibody

Anti-AKT (phospho Tyr315) antibody used in Western Blot (WB). GTX50869
Anti-AKT (phospho Tyr315) antibody used in Western Blot (WB). GTX50869
Anti-AKT (phospho Tyr315) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50869
Anti-AKT (phospho Tyr315) antibody used in IHC (Paraffin sections) (IHC-P). GTX50869

Cat. No. GTX50869

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all AKT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Product Note

The antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT serine/threonine kinase 2 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Membrane

Database

Research Area

DATA IMAGES

Anti-AKT (phospho Tyr315) antibody used in Western Blot (WB). GTX50869

GTX50869 WB Image

WB analysis of extracts from HepG2 cells untreated or treated with EGF using GTX50869 AKT (phospho Tyr315) antibody.

Anti-AKT (phospho Tyr315) antibody used in Western Blot (WB). GTX50869

GTX50869 WB Image

WB analysis of extracts from 3T3 cells treated with EGF or calf intestinal phosphatase (CIP) using GTX50869 AKT (phospho Tyr315) antibody.

Anti-AKT (phospho Tyr315) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50869

GTX50869 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50869 AKT (phospho Tyr315) antibody.

Anti-AKT (phospho Tyr315) antibody used in IHC (Paraffin sections) (IHC-P). GTX50869

GTX50869 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50869 AKT (phospho Tyr315) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399