GeneTex
United States (US)

AKT2 (active) recombinant protein

Cat No. GTX65003

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

46 nmol phosphate incorporated into Akt/SGK substrate (RPRAATF) per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP and total of 0.83 uCi/ul P-32.

Calculated MW

58.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥90%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKT2, Akt, RAC-alpha serine/threonine-protein kinase, RACalpha serine/threonineprotein kinase

Background

Akt2 or Protein Kinase B beta (PKBbeta) is a serine/threonine kinase that is a member of the Akt family. Akt2 like the other Akt members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3, 4, 5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2. Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (3).

Research Area

Package List Price ($)
$ 359