APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , AKT3-transfected 293T cells
Product Note
Based on internal testing result, this antibody may cross react with AKT1 protein.
Predict Reactivity
Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.15 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Akt3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036200
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AKT serine/threonine kinase 3 , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2
Cellular Localization
Cytoplasm , Membrane
Background
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113312 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT3 antibody (GTX113312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX113312 WB Image
Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX113312 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113312 IHC-P Image
AKT3 antibody detects AKT3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.AKT3 stained by AKT3 antibody (GTX113312) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113312 ICC/IF Image
AKT3 antibody detects AKT3 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AKT3 stained by AKT3 antibody (GTX113312) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX113312 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT3 antibody (GTX113312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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