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ALAD antibody, C-term

Anti-ALAD antibody, C-term used in Flow cytometry (FACS). GTX81701
Anti-ALAD antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81701
Anti-ALAD antibody, C-term used in Western Blot (WB). GTX81701

Cat. No. GTX81701

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all ALAD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 244-272 amino acids from the C-terminal region of human ALAD.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11178918

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aminolevulinate dehydratase , ALADH , PBGS

Background

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-ALAD antibody, C-term used in Flow cytometry (FACS). GTX81701

GTX81701 FACS Image

FACS analysis of Jurkat cells using GTX81701 ALAD antibody, C-term.
Green : primary antibody
Blue : negative control

Anti-ALAD antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81701

GTX81701 IHC-P Image

IHC-P analysis of human brain tissue using GTX81701 ALAD antibody, C-term.

Anti-ALAD antibody, C-term used in Western Blot (WB). GTX81701

GTX81701 WB Image

WB analysis of Jurkat cell lysate (35ug/lane) using GTX81701 ALAD antibody, C-term.

REFERENCE

Application Reference

Ast T et al. Cell 2019; 177 (6) : 1507-1521.e16 Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399