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ALDH1A2 antibody [C1C3]

Anti-ALDH1A2 antibody [C1C3] used in IHC (Whole mount) (IHC-Wm). GTX115148
Anti-ALDH1A2 antibody [C1C3] used in IHC (Whole mount) (IHC-Wm). GTX115148
Anti-ALDH1A2 antibody [C1C3] used in Western Blot (WB). GTX115148
Anti-ALDH1A2 antibody [C1C3] used in Western Blot (WB). GTX115148
Anti-ALDH1A2 antibody [C1C3] used in Western Blot (WB). GTX115148
Anti-ALDH1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115148
Anti-ALDH1A2 antibody [C1C3] used in Western Blot (WB). GTX115148

Cat. No. GTX115148

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ALDH1A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Mouse liver , IMR32 , rat testis , mouse testis

Predict Reactivity

Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619235

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member A2 , RALDH(II) , RALDH2 , RALDH2-T

Cellular Localization

Cytoplasm

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALDH1A2 antibody [C1C3] used in IHC (Whole mount) (IHC-Wm). GTX115148

GTX115148 IHC-Wm Image

ALDH1A2 antibody [C1C3] detects Aldh1a2 protein at dorsal retina on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
ALDH1A2 antibody [C1C3] (GTX115148) dilution: 1:200.
Anti-ALDH1A2 antibody [C1C3] used in IHC (Whole mount) (IHC-Wm). GTX115148

GTX115148 IHC-Wm Image

ALDH1A2 antibody [C1C3] detects Aldh1a2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
ALDH1A2 antibody [C1C3] (GTX115148) dilution: 1:100.

Anti-ALDH1A2 antibody [C1C3] used in Western Blot (WB). GTX115148

GTX115148 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX115148 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A2 antibody [C1C3] used in Western Blot (WB). GTX115148

GTX115148 WB Image

ALDH1A2 antibody detects ALDH1A2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody (GTX115148) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A2 antibody [C1C3] used in Western Blot (WB). GTX115148

GTX115148 WB Image

Sample (30 μg of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX115148 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115148

GTX115148 IHC-P Image

ALDH1A2 antibody [C1C3] detects ALDH1A2 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
ALDH1A2 antibody [C1C3] (GTX115148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALDH1A2 antibody [C1C3] used in Western Blot (WB). GTX115148

GTX115148 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody (GTX115148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169