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ALDH2 antibody

KO/KD Orthogonal
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in ELISA (ELISA). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in IHC (Whole mount) (IHC-Wm). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

Cat. No. GTX101429

Reference 7
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm, ELISA, IHC, Sandwich ELISA

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ALDH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
ELISA 1:1000-1:10000
IHC Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX101429 , Detection: GTX88073
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

GL261 , C8D30 , BCL-1 , Raw264.7

Predict Reactivity

Rat, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949628

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 2 family member , ALDH-E2 , ALDHI , ALDM

Cellular Localization

Mitochondrion matrix

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

ALDH2 antibody detects ALDH2 protein at mitochondria on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ALDH2 antibody (GTX101429) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

ALDH2 antibody detects ALDH2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) at a dilution of 1:500.

Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

Orthogonal

HepG2 whole cell and cytoplasm extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) diluted at 1:500.

Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

ALDH2 antibody detects ALDH2 protein by western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysae/extract
D. 30 μg Raw264.7 whole cell lysate/extract
7.5% SDS-PAGE
ALDH2 antibody (GTX101429) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

ALDH2 antibody detects ALDH2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ALDH2 antibody (GTX101429) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH2 antibody used in ELISA (ELISA). GTX101429

GTX101429 ELISA Image

ELISA detection of ALDH2 using GTX101429 for capture at a concentration of 2.5 μg/mL and GTX88073 for detection at a concentration of 1.5 μg/mL.

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ALDH2(GTX101429) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH2 antibody used in IHC (Whole mount) (IHC-Wm). GTX101429

GTX101429 IHC-Wm Image

ALDH2 antibody detects ALDH2 protein at mitochondria on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
ALDH2 antibody (GTX101429) dilution: 1:200.
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

ALDH2 antibody detects ALDH2 protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) at a dilution of 1:2000.

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH2 antibody (GTX101429) at 1:300 dilution.

REFERENCE

Application Reference

Lin YH et al. J Cancer 2023; 14 (10) : 1689-1706 Analysis of Aldehyde Dehydrogenase 2 as a Prognostic Marker Associated with Immune Cell infiltration and Chemotherapy Efficacy in Head and Neck Squamous Cell Carcinoma.
Yu-Hsuan Lin et al. J. Cancer 2023; 14 (10) : 1689-1706 Analysis of Aldehyde Dehydrogenase 2 as a Prognostic Marker Associated with Immune Cell infiltration and Chemotherapy Efficacy in Head and Neck Squamous Cell Carcinoma
Application : WB, IHC
Reactivity : Human
Masato A et al. NPJ Parkinsons Dis 2023; 9 (1) : 42 DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.
Application : WB
Shiau-Mei Chen et al. Biomolecules 2021; 11 (10) Activation of Aldehyde Dehydrogenase 2 Ameliorates Glucolipotoxicity of Pancreatic Beta Cells.
Application : WB
Reactivity : Mouse
Kitakaze T et al. Arch Biochem Biophys 2020; 686 : 108329 6-(Methylsulfinyl)hexyl isothiocyanate protects acetaldehyde-caused cytotoxicity through the induction of aldehyde dehydrogenase in hepatocytes.
Application : WB
Reactivity : Mouse
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REVIEW

ALDH2 antibody Cat. No. GTX101429

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : MIN6 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169