APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 1-3μg/ml |
|
Note :
IHC-P
Human Testis shows staining of cytoplasm in spermatocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX24306-PEP).
Product Note
The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RVTNQLLGRKVPWD, from the C Terminus of the protein sequence according to NP_055935.4.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ALMS1 centrosome and basal body associated protein , ALSS
Cellular Localization
Cytoplasm
Background
This gene encodes a protein containing a large tandem-repeat domain as well as additional low complexity regions. The encoded protein functions in microtubule organization, particularly in the formation and maintanance of cilia. Mutations in this gene cause Alstrom syndrome. There is a pseudogene for this gene located adjacent in the same region of chromosome 2. Alternative splice variants have been described but their full length nature has not been determined. [provided by RefSeq, Apr 2014]
Database
Research Area
DATA IMAGES
|
GTX24306 ICC/IF Image
ICC/IF analysis of paraformaldehyde fixed HepG2 cells using GTX24306 ALMS1 antibody.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml
|
|
|
GTX24306 ICC/IF Image
ICC/IF analysis of paraformaldehyde fixed HeLa cells using GTX24306 ALMS1 antibody.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml
|
|
|
GTX24306 IHC-P Image
IHC-P analysis of human testis using GTX24306 ALMS1 antibody.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml
|
REFERENCE
There are currently no references for ALMS1 antibody (GTX24306). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ALMS1 antibody (GTX24306). Be the first to share your experience with this product.
