GeneTex

  • Country / Location Selection

United States (US)

ALY antibody

Anti-ALY antibody used in IHC (Paraffin sections) (IHC-P). GTX113917
Anti-ALY antibody used in IHC (Paraffin sections) (IHC-P). GTX113917
Anti-ALY antibody used in Western Blot (WB). GTX113917
Anti-ALY antibody used in Western Blot (WB). GTX113917
Anti-ALY antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113917

Cat. No. GTX113917

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ALY products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLa , HepG2

Product Note

Knockdown/Knockout validation was supported by references (PMID: 33991457).

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALY. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036218

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Aly/REF export factor , ALY , ALY/REF , BEF , REF , THOC4

Cellular Localization

Nucleus , Nucleus speckle , Cytoplasm

Background

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALY antibody used in IHC (Paraffin sections) (IHC-P). GTX113917

GTX113917 IHC-P Image

ALY antibody detects ALY protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ALY antibody (GTX113917) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALY antibody used in IHC (Paraffin sections) (IHC-P). GTX113917

GTX113917 IHC-P Image

ALY antibody detects ALY protein at nucleus in human esophageal cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal cancer.
ALY antibody (GTX113917) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALY antibody used in Western Blot (WB). GTX113917

GTX113917 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113917 diluted at 1:1000

Anti-ALY antibody used in Western Blot (WB). GTX113917

GTX113917 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ALY antibody (GTX113917) diluted at 1:500.

Anti-ALY antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113917

GTX113917 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ALY(GTX113917) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

Application Reference

Jing-Zi Wang et al. Cancer Commun (Lond) 2021; 41 (7) : 560-575 The role of the HIF-1α/ALYREF/PKM2 axis in glycolysis and tumorigenesis of bladder cancer.
Application : WB
Reactivity : Human
Huang HC et al. J Biol Chem 2016; (Epub) Cellular Nuclear Export Factors TAP and Aly Are Required for HDAg-L-mediated Assembly of Hepatitis Delta Virus.
Application : WB, IP
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF, IP
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for ALY antibody (GTX113917). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169