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AMPK alpha 1 + AMPK alpha 2 antibody

Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50863
Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50863
Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in Western Blot (WB). GTX50863

Cat. No. GTX50863

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all AMPK alpha 1 + AMPK alpha 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 181~185/170~174 (L-R-T-S-C) derived from human AMPK alpha 1/2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168457

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , protein kinase AMP-activated catalytic subunit alpha 2 , AMPK , AMPKa1 , AMPK , AMPK2 , AMPKa2 , PRKAA

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50863

GTX50863 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50863 AMPK alpha 1/2 antibody.

Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50863

GTX50863 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50863 AMPK alpha 1/2 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in Western Blot (WB). GTX50863

GTX50863 WB Image

WB analysis of extracts from A549, HepG2, and 3T3 cells using GTX50863 AMPK alpha 1/2 antibody.

REFERENCE

Application Reference

Lee S et al. Front Pharmacol 2021; 12 : 747975 HEXA-018, a Novel Inducer of Autophagy, Rescues TDP-43 Toxicity in Neuronal Cells.
Application : WB
Reactivity : Mouse
Irungbam K et al. Lab Invest 2020; 100 (11) : 1411-1424 Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice.
Application : WB
Reactivity : Mouse
Irungbam K et al. Lab Invest 2019; Cannabinoid receptor 1 knockout alleviates hepatic steatosis by downregulating perilipin 2.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399