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AMPK alpha 1 (phospho Ser496) antibody

Orthogonal
Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX50224
Anti-AMPK alpha 1 (phospho Ser496) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50224
Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX50224
Anti-AMPK alpha 1 (phospho Ser496) antibody used in IHC (Paraffin sections) (IHC-P). GTX50224

Cat. No. GTX50224

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all AMPK alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 487 (S-G- S(P)-V-S) derived from human AMPK alpha 1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , AMPK , AMPKa1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX50224

GTX50224 WB Image

Orthogonal

WB analysis of extracts from HUVEC cells untreated or treated with UV using GTX50224 AMPK alpha 1 (phospho Ser496) antibody.

Anti-AMPK alpha 1 (phospho Ser496) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50224

GTX50224 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50224 AMPK alpha 1 (phospho Ser496) antibody.

Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX50224

GTX50224 WB Image

WB analysis of extracts from C6 cells treated with EGF or calf intestinal phosphatase (CIP) using GTX50224 AMPK alpha 1 (phospho Ser496) antibody.

Anti-AMPK alpha 1 (phospho Ser496) antibody used in IHC (Paraffin sections) (IHC-P). GTX50224

GTX50224 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX50224 AMPK alpha 1 (phospho Ser496) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

There are currently no reviews for AMPK alpha 1 (phospho Ser496) antibody (GTX50224). Be the first to share your experience with this product.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399