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AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody

Orthogonal
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX130429
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX130429

Cat. No. GTX130429

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AMPK alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Product Note

The sequence around AMPK alpha 1 (phospho Thr183) is conserved with the sequence around AMPK alpha 2 (phospho Thr172).

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr183 of human AMPK alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886265

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , AMPK , AMPKa1

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX130429

GTX130429 WB Image

Orthogonal

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX130429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX130429

GTX130429 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX130429) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Suzuki K et al. J Biol Chem 2023; 299 (10) : 105210 CLPX regulates mitochondrial fatty acid β-oxidation in liver?cells.
Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Lee S et al. Front Pharmacol 2021; 12 : 747975 HEXA-018, a Novel Inducer of Autophagy, Rescues TDP-43 Toxicity in Neuronal Cells.
Application : WB
Reactivity : Mouse
Jiang WP et al. Antioxidants (Basel) 2021; 10 (6) Sanghuangporus sanghuang Mycelium Prevents Paracetamol-Induced Hepatotoxicity through Regulating the MAPK/NF-κB, Keap1/Nrf2/HO-1, TLR4/PI3K/Akt, and CaMKKβ/LKB1/AMPK Pathways and Suppressing Oxidative Stress and Inflammation.
Application : WB
Reactivity : Mouse
Irungbam K et al. Lab Invest 2020; 100 (3) : 454-465 Cannabinoid receptor 1 knockout alleviates hepatic steatosis by downregulating perilipin 2.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169