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AMPK beta 1 antibody

KO/KD
Anti-AMPK beta 1 antibody used in Western Blot (WB). GTX57162
Anti-AMPK beta 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57162
Anti-AMPK beta 1 antibody used in Western Blot (WB). GTX57162
Anti-AMPK beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX57162

Cat. No. GTX57162

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all AMPK beta 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human PRKAB1 (NP_006244.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated non-catalytic subunit beta 1 , AMPK , HAMPKb

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK beta 1 antibody used in Western Blot (WB). GTX57162

GTX57162 WB Image

WB analysis of various sample lysates using GTX57162 AMPK beta 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-AMPK beta 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57162

GTX57162 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX57162 AMPK beta 1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-AMPK beta 1 antibody used in Western Blot (WB). GTX57162

GTX57162 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX57162 AMPK beta 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-AMPK beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX57162

GTX57162 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX57162 AMPK beta 1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399