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ARD1A antibody

KO/KD
Anti-ARD1A antibody used in Western Blot (WB). GTX125971
Anti-ARD1A antibody used in Western Blot (WB). GTX125971
Anti-ARD1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125971
Anti-ARD1A antibody used in Immunoprecipitation (IP). GTX125971
Anti-ARD1A antibody used in IHC (Paraffin sections) (IHC-P). GTX125971

Cat. No. GTX125971

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ARD1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

H1299 transfected scramble siRNA , H1299 transfected ARDIA siRNA , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ARD1A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166967

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N(alpha)-acetyltransferase 10, NatA catalytic subunit , ARD1 , ARD1A , ARD1P , DXS707 , MCOPS1 , NATD , OGDNS , TE2 , hARD1

Cellular Localization

Cytoplasm , Nucleus

Background

N-alpha-acetylation is one of the most common protein modifications that occurs during protein synthesis and involves the transfer of an acetyl group from acetyl-coenzyme A to the protein alpha-amino group. ARD1A, together with NATH (NARG1; MIM 608000), is part of a major N-alpha-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides (Sanchez-Puig and Fersht, 2006 [PubMed 16823041]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ARD1A antibody used in Western Blot (WB). GTX125971

GTX125971 WB Image

KO/KD

Sample (30 μg of whole cell lysate)
A: H1299 transfected scramble siRNA
B: H1299 transfected ARDIA siRNA
12% SDS PAGE
GTX125971 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARD1A antibody used in Western Blot (WB). GTX125971

GTX125971 WB Image

ARD1A antibody detects ARD1A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and blotted with ARD1A antibody (GTX125971) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARD1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125971

GTX125971 ICC/IF Image

ARD1A antibody detects NAA10 protein at Cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min.
Green: NAA10 protein stained by ARD1A antibody (GTX125971) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-ARD1A antibody used in Immunoprecipitation (IP). GTX125971

GTX125971 IP Image

Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 μg of ARD1A antibody (GTX125971).
Western blot analysis was performed using ARD1A antibody (GTX125971) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ARD1A antibody used in IHC (Paraffin sections) (IHC-P). GTX125971

GTX125971 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using ARD1A(GTX125971) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Jiang Y et al. Cancer Sci 2020; 111 (5) : 1829-1839 Metagenomic characterization of lysine acetyltransferases in human cancer and their association with clinicopathologic features.
Application : WB
Reactivity : Human
Takekoshi D et al. In Vitro Cell Dev Biol Anim 2019; 55 (5) : 355-367 The N-end rule pathway enzyme Naa10 supports epiblast specification in mouse embryonic stem cells by modulating FGF/MAPK.
Application : WB
Reactivity : Mouse
Owen J et al. PLoS One 2016; 11 (12) : e0168088 Reducing Tumour Hypoxia via Oral Administration of Oxygen Nanobubbles.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169