GeneTex
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ATF2 (phospho Thr69) antibody

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX32317
Anti-ATF2 (phospho Thr69) antibody used in Western Blot (WB). GTX32317

Cat No. GTX32317

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of ATF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Activating Transcription Factor 2,Cre-Bp1,Creb-2,Creb2,Hb16,Treb7,Atf2

Cellular Localization

Cytoplasm,Mitochondrion outer membrane,Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX32317

GTX32317 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX32317 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-ATF2 (phospho Thr69) antibody used in Western Blot (WB). GTX32317

GTX32317 WB Image

WB analysis of HeLa (A), NIH3T3 (B), PC12 (C) whole cell lysates using GTX32317 ATF2 (phospho Thr69) antibody.

Package List Price ($)
$ 319