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ATR (phospho Thr1989) antibody

GTX128145_42109_20171020_WB_Fraction_competitor_watermark_18121410_879.jpg
GTX128145_42593_20161116_IHC-P_H_2_18121410_802.jpg
GTX128145_42593_20161116_IHC-P_H_18121410_461.jpg
GTX128145_42347_20160127_IHC-P_M_2_18121410_989.jpg
GTX128145_42347_20160127_IHC-P_M_1_18121410_215.jpg
GTX128145_43089_20180213_IHC-P_1_18121410_923.jpg
GTX128145_42179_20151210_WB_Fraction_18121410_551.jpg
GTX128145_43089_20180213_IHC-P_18121410_101.jpg
GTX128145_43145_20180506_ICC_IF_18121410_594.jpg
GTX128145_41675_20150317_IP_18121410_370.jpg
GTX128145_42592_20161110_IHC-P_H_18121410_347.jpg
GTX128145_42396_20160310_WB_Hydroxyurea_18121410_531.jpg
GTX128145_42396_20160420_IHC-P_H_18121410_389.jpg

Cat No. GTX128145

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

301 kDa. ( Note )

Positive Control

HCT116 , HCT116 treated 1mM Hydroxyurea , Raji , Raji nuclear extract
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human ATR (phospho Thr1989). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atr Serine/Threonine Kinase,Fctcs,Frp1,Mec1,Sckl,Sckl1,Atr

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX128145_42109_20171020_WB_Fraction_competitor_watermark_18121410_879.jpg

GTX128145 WB Image

Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500 and competitor's antibody (CST#2790) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX128145_42593_20161116_IHC-P_H_2_18121410_802.jpg

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128145_42593_20161116_IHC-P_H_18121410_461.jpg

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128145_42347_20160127_IHC-P_M_2_18121410_989.jpg

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128145_42347_20160127_IHC-P_M_1_18121410_215.jpg

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128145_43089_20180213_IHC-P_1_18121410_923.jpg

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128145_42179_20151210_WB_Fraction_18121410_551.jpg

GTX128145 WB Image

Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128145_43089_20180213_IHC-P_18121410_101.jpg

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128145_43145_20180506_ICC_IF_18121410_594.jpg

GTX128145 ICC/IF Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX128145_41675_20150317_IP_18121410_370.jpg

GTX128145 IP Image

Immunoprecipitation of ATR (phospho Thr1989) protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 μg of ATR (phospho Thr1989) antibody (GTX128145).
Western blot analysis was performed using ATR (phospho Thr1989) antibody (GTX128145).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX128145_42592_20161110_IHC-P_H_18121410_347.jpg

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR protein at nucleus in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128145_42396_20160310_WB_Hydroxyurea_18121410_531.jpg

GTX128145 WB Image

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:1000.

GTX128145_42396_20160420_IHC-P_H_18121410_389.jpg

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human lung by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

ATR (phospho Thr1989) antibody Cat No. GTX128145

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Feb-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Amount used : 20μg
Blocking : 5% milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf