GeneTex

  • Country / Location Selection

United States (US)

Abin 2 antibody [N1C2]

Anti-Abin 2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108962
Anti-Abin 2 antibody [N1C2] used in Western Blot (WB). GTX108962
Anti-Abin 2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX108962

Cat. No. GTX108962

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Abin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

A431 , HeLaS3

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Abin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036143

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNFAIP3 interacting protein 2 , ABIN2 , FLIP1 , KLIP

Cellular Localization

Cytoplasm

Background

TNIP2 binds to the C-terminal zinc finger domain of A20 (TNFAIP3; MIM 191163) and is involved in activation of the ERK (see MAPK3; MIM 601795) MAP kinase pathway in various cell types (Van Huffel et al., 2001 [PubMed 11390377]; Papoutsopoulou et al., 2006 [PubMed 16633345]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Abin 2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108962

GTX108962 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Abin-2 (GTX108962) antibody at 1:200 dilution.

Anti-Abin 2 antibody [N1C2] used in Western Blot (WB). GTX108962

GTX108962 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela S3
10% SDS PAGE
GTX108962 diluted at 1:1000

Anti-Abin 2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX108962

GTX108962 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using Abin-2 (GTX108962) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Chen JY et al. J Nutr Biochem 2013; 24 (11) : 1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Abin 2 antibody [N1C2] (GTX108962). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169