ELISA: Use at a dilution of 1/2000 - 1/5000. Optimal dilutions/concentrations should be determined by the end user.
Using a conjugate Acrolein Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds.Acrolein-BSA (ratio 1:1), Malondialdehyde-BSA (1:55), Glyoxal-BSA (1:> 50,000), Formaldehyde-BSA (1:> 50,000), Glutaraldehyde-BSA (1/> 50,000). Crossreactivity ratio is the Acrolein BSA concentration/unconjugated or conjugated analogs concentration at half displacement.
Preservative: NoneConstituents: 40% Glycerol, PBS
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Acrolein, a reactive alpha,beta-unsaturated aldehyde, is a common environmental pollutant, a metabolite of the anticancer drug cyclophosphamide, and a byproduct of lipid peroxidation. An increase in acrolein production has been proposed as a marker for Alzheimer's disease, diabetic glomerular lesions, and atherosclerosis. Acrolein is a potent inhibitor of cell proliferation at nonlethal doses and may act through effects on redox-regulated transcription factors. It has been reported that NF-kappaB activation is inhibited by acrolein.