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Adiponectin antibody

Orthogonal
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in IHC (Paraffin sections) (IHC-P). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

Cat. No. GTX112777

Reference 20
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Plant
Package
100 μl ($399),
25 μl ($169)
See all Adiponectin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Human plasma , Mouse plasma , rat plasma

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Adiponectin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885323

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adiponectin, C1Q and collagen domain containing , ACDC , ACRP30 , ADIPQTL1 , ADPN , APM-1 , APM1 , GBP28

Cellular Localization

Secreted

Background

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Anti-Adiponectin antibody used in IHC (Paraffin sections) (IHC-P). GTX112777

GTX112777 IHC-P Image

Adiponectin antibody detects Adiponectin protein at cytoplasm and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Adiponectin stained by Adiponectin antibody (GTX112777) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Adiponectin antibody detects Adiponectin protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

REFERENCE

Application Reference

Felix JB et al. Res Sq 2024; N-acetylaspartate from fat cells regulates postprandial body temperature.
Abgrall A et al. Cells 2022; 11 (17) Evidence for the Neuronal Expression and Secretion of Adiponectin.
Wen S et al. Int J Mol Sci 2022; 23 (19) Contraction Band Necrosis with Dephosphorylated Connexin 43 in Rat Myocardium after Daily Cocaine Administration.
Application : WB
Reactivity : Rat
Tews D et al. Int J Obes (Lond) 2022; 46 (11) : 1939-1947 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biology.
Application : WB
Reactivity : Human
Cox AR et al. Cell Metab 2022; 34 (12) : 1932-1946.e7 The rheumatoid arthritis drug auranofin lowers leptin levels and exerts antidiabetic effects in obese mice.
Show More +
Submit a Reference

REVIEW

Adiponectin antibody Cat. No. GTX112777

Rating
( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 4 users reviews)
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Date : Anonymous submitted on 27-Dec-2020
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat plasma
Amount used : 5 μg
Blocking : 2% skimmed milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Date : Anonymous submitted on 23-Dec-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : white preadipocytes cells
Amount used : 10μg
Blocking : 5% w/v nonfat milk in TBST
Primary Antibody : 1:1000, 25°C, 2Hr
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Date : Anonymous submitted on 26-Aug-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 120μg
Blocking : 5% skim milk, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Submit a Review
Application Reference
Felix JB et al. Res Sq 2024; N-acetylaspartate from fat cells regulates postprandial body temperature.
Abgrall A et al. Cells 2022; 11 (17) Evidence for the Neuronal Expression and Secretion of Adiponectin.
Wen S et al. Int J Mol Sci 2022; 23 (19) Contraction Band Necrosis with Dephosphorylated Connexin 43 in Rat Myocardium after Daily Cocaine Administration.
Application : WB
Reactivity : Rat
Tews D et al. Int J Obes (Lond) 2022; 46 (11):1939-1947 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biology.
Application : WB
Reactivity : Human
Cox AR et al. Cell Metab 2022; 34 (12):1932-1946.e7 The rheumatoid arthritis drug auranofin lowers leptin levels and exerts antidiabetic effects in obese mice.
Saveta G Mladenova et al. Front Pharmacol 2021; 12:707507 Anti-Adipogenic Effect of Alchemilla monticola is Mediated Via PI3K/AKT Signaling Inhibition in Human Adipocytes.
Application : WB
Reactivity : Human
Wu H et al. Cells 2021; 10 (11) microRNA-27a-3p but Not -5p Is a Crucial Mediator of Human Adipogenesis.
Application : WB
Reactivity : Human
Savova MS et al. Biomed Pharmacother 2021; 141:111934 Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.
Application : WB
Reactivity : Plant
Cox AR et al. Mol Metab 2021; 48:101221 Ube2i deletion in adipocytes causes lipoatrophy in mice.
Application : WB
Vasileva LV et al. Food Chem Toxicol 2021; 149:112002 Rosmarinic acid attenuates obesity and obesity-related inflammation in human adipocytes.
Application : WB
Reactivity : Human
Liliya V Vasileva et al. Int J Mol Sci 2020; 21 (24) Caffeic and Chlorogenic Acids Synergistically Activate Browning Program in Human Adipocytes: Implications of AMPK- and PPAR-Mediated Pathways.
Application : WB
Reactivity : Human
Roos J et al. Cell Mol Life Sci 2020; (Epub) miR-146a regulates insulin sensitivity via NPR3.
Application : WB
Reactivity : Mouse
Saha PK et al. Am J Physiol Endocrinol Metab 2020; 319 (4):E667-E677 miR-30a targets gene networks that promote browning of human and mouse adipocytes.
Application : WB
Reactivity : Human
Chernis N et al. Am J Physiol Cell Physiol 2019; Bisphenol AF promotes inflammation in human white adipocytes.
Application : WB
Reactivity : Human
Tews D et al. Redox Biol 2019; 26:101286 Elevated UCP1 levels are sufficient to improve glucose uptake in human white adipocytes.
Application : WB
Reactivity : Mouse
Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.
Application : WB
Reactivity : Mouse
Chou CL et al. PLoS One 2017; 12 (7):e0180712 Renin inhibition improves metabolic syndrome, and reduces angiotensin II levels and oxidative stress in visceral fat tissues in fructose-fed rats.
Application : WB
Reactivity : Rat
Schendel SA et al. Aesthet Surg J 2017; (Epub) Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.
Application : WB
Reactivity : Human
Tsai JR et al. PLoS One 2015; 10 (12):e0144462 Curcumin Inhibits Non-Small Cell Lung Cancer Cells Metastasis through the Adiponectin/NF-κb/MMPs Signaling Pathway.
Application : WB, IHC-P
Reactivity : Human
Hartig SM et al. Mol Endocrinol 2015; 29 (9):1320-33 Ubc9 Impairs Activation of the Brown Fat Energy Metabolism Program in Human White Adipocytes.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169