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Alpha fetoprotein / AFP antibody

Anti-Alpha fetoprotein / AFP antibody used in Western Blot (WB). GTX30030
Anti-Alpha fetoprotein / AFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30030

Cat. No. GTX30030

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Alpha fetoprotein / AFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human AFP

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha fetoprotein , AFPD , FETA , HPAFP

Cellular Localization

Secreted

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody used in Western Blot (WB). GTX30030

GTX30030 WB Image

WB analysis of various sample lysates using GTX30030 Alpha fetoprotein / AFP antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Alpha fetoprotein / AFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30030

GTX30030 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30030 Alpha fetoprotein / AFP antibody.
Blue : DAPI

REFERENCE

Application Reference

Wei Shan et al. Exp Ther Med 2021; 21 (6) : 619 Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399