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Androgen Receptor (phospho Ser213) antibody

Orthogonal
Anti-Androgen Receptor (phospho Ser213) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50182
Anti-Androgen Receptor (phospho Ser213) antibody used in Western Blot (WB). GTX50182

Cat. No. GTX50182

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Androgen Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 213 (E-A-S(p)-G-A) derived from human Androgen Receptor.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11172028

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM

Cellular Localization

Nucleus,Cytoplasm

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES

Anti-Androgen Receptor (phospho Ser213) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50182

GTX50182 ICC/IF Image

Orthogonal

ICC/IF analysis of methanol-fixed HeLa cells using GTX50182 Androgen Receptor (phospho Ser213) antibody.

Anti-Androgen Receptor (phospho Ser213) antibody used in Western Blot (WB). GTX50182

GTX50182 WB Image

WB analysis of extracts from DU145 cells using GTX50182 Androgen Receptor (phospho Ser213) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Kuo YY et al. Cell Commun Signal 2019; 17 (1) : 100 Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81 and Ser213.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399