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Annexin V antibody

KO/KD
Anti-Annexin V antibody used in Western Blot (WB). GTX103250
Anti-Annexin V antibody used in Western Blot (WB). GTX103250
Anti-Annexin V antibody used in Flow cytometry (FACS). GTX103250
Anti-Annexin V antibody used in Western Blot (WB). GTX103250
Anti-Annexin V antibody used in Western Blot (WB). GTX103250
Anti-Annexin V antibody used in Western Blot (WB). GTX103250
Anti-Annexin V antibody used in Western Blot (WB). GTX103250

Cat. No. GTX103250

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Annexin V products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:50000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

PC-12 , Rat2 , A431 , HeLa , HepG2 , GL261 , C8D30 , NIH-3T3 , Raw 264.7 , 293T

Predict Reactivity

Bovine, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin V. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949660

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A5 , ANX5 , ENX2 , HEL-S-7 , PP4 , RPRGL3

Background

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

KO/KD

Wild-type (WT) and Annexin V knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody (GTX103250) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody used in Flow cytometry (FACS). GTX103250

GTX103250 FACS Image

Annexin V antibody (GTX103250) detects Annexin V protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: Annexin V antibody (GTX103250) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis. A. 30 μg GL261 whole cell lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract 10% SDS-PAGE Annexin V antibody (GTX103250) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody (GTX103250) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

The data was published in the 2022 in Front Cell Dev Biol. PMID: 35573689

REFERENCE

Application Reference

Butts B et al. Front Cardiovasc Med 2023; 10 : 1132786 Impact of early pericardial fluid chymase activation after cardiac surgery.
Ursula S Sandau et al. Front Cell Dev Biol 2022; 10 : 864022 Differential Effects of APOE Genotype on MicroRNA Cargo of Cerebrospinal Fluid Extracellular Vesicles in Females With Alzheimer's Disease Compared to Males.
Application : WB
Reactivity : Human
Thangavel C et al. Clin Cancer Res 2018; (Epub) Therapeutic Challenge With a CDK 4/6 Inhibitor Induces an RB-dependent SMAC Mediated Apoptotic Response in Non-Small Cell Lung Cancer.
Application : WB
Reactivity : Human
Dong RF et al. J Cell Biochem 2017; (Epub) The Neuroprotective Role of MiR-124-3p in a 6-Hydroxydopamine-Induced Cell Model of Parkinson's Disease via the Regulation of ANAX5.
Application : WB
Reactivity : Human, Rat
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Butts B et al. Front Cardiovasc Med 2023; 10:1132786 Impact of early pericardial fluid chymase activation after cardiac surgery.
Ursula S Sandau et al. Front Cell Dev Biol 2022; 10:864022 Differential Effects of APOE Genotype on MicroRNA Cargo of Cerebrospinal Fluid Extracellular Vesicles in Females With Alzheimer's Disease Compared to Males.
Application : WB
Reactivity : Human
Thangavel C et al. Clin Cancer Res 2018; (Epub) Therapeutic Challenge With a CDK 4/6 Inhibitor Induces an RB-dependent SMAC Mediated Apoptotic Response in Non-Small Cell Lung Cancer.
Application : WB
Reactivity : Human
Dong RF et al. J Cell Biochem 2017; (Epub) The Neuroprotective Role of MiR-124-3p in a 6-Hydroxydopamine-Induced Cell Model of Parkinson's Disease via the Regulation of ANAX5.
Application : WB
Reactivity : Human, Rat
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Chen JY et al. J Nutr Biochem 2013; 24 (11):1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : WB
Reactivity : Human
Ruggiero L et al. Proc Natl Acad Sci U S A 2012; 109 (21):2281154 Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169