GeneTex
United States (US)

Antithrombin III antibody

Cat No. GTX28776

Host Sheep
Clonality Polyclonal
Isotype IgG
Application DID, IE, RID
Reactivity Human, Mouse, Rat, Cat, Dog, Horse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
DID Assay dependent
IE Assay dependent
RID Assay dependent

Note :

DID
10 μl antiserum vs. 5 μl plasma

IE
100 μl antiserum vs. 5 μl plasma

RID
6 μl antiserum / cm2 gel vs. 5 μl neat - 1/10 dilution human plasma

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Specificity/Sensitivity

Antithrombin III (ATH III) is a plasma protein synthesized in the liver. Normal plasma levels are115-160 mg/L. ATH III consists of a single polypeptide chain and has a molecular weight of 58 kDa.ATH III is a serine protease inhibitor and through this action regulates blood coagulation. ATH III also has a heparinbinding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000-fold.Gives a single arc when tested by IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony)against human plasma and a known anti-human antithrombin III. The cross reactivity of this product against the sera ofthe species listed below has been assessed by double diffusion using 10 μL of antisera against 5 μL of species sera.These results are intended as a guideline only. It is possible that cross-reactivity against species listed as negative maybe observed when using different test conditions or techniques.CAT + CHICKEN - COW - DOG + G.PIG - GOAT -HORSE + HUMAN + MOUSE + PIG - RABBIT - RAT +
PROPERTIES

Form

Liquid

Buffer

Glycine Buffered Saline pH 7.4, 0.099% sodium azide, 0.1% EACA, 0.01% Benzamidine, 1 mM EDTA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

14.30 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human antithrombin III purified from plasma, shown to be homogeneous by SDS-PAGE.

Purification

IgG fraction
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serpin Family C Member 1,At3,At3D,Atiii,Atiii-R2,Thph7,Serpinc1

Background

The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]

Database

Research Area

REFERENCE
Package List Price ($)
$ 339