20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, 0.5% Tween 20, and 0.5% Nonidet P40.
Store at -80ºC. Product is stable for at least 12 months.
2.5U/μl(Please refer to the vial label for the specific concentration.)
Purity was assessed by SDS-PAGE and by HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Taq DNA Polymerase is a thermostable enzyme that replicates DNA and exhibits a half-life of 40 minutes at 95ºC. Taq catalyzes the polymerization of nucleotides into duplex DNA in the 5’ –3’ direction in the presence of magnesium. The enzyme has an apparent molecular weight of 94,000 daltons by SDS-PAGE and exhibits 5’– 3’exonuclease activity. Taq is recommended for use in PCR and primer extension reactions at elevated temperature. The 10X Reaction Buffer provided has been optimized for amplification of both short and long PCR products.