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Ataxin 3 antibody

Orthogonal Overexpression
Anti-Ataxin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115032
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032

Cat. No. GTX115032

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Ataxin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

mouse brain , human Ataxin3-transfected 293T

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ataxin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621230

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ataxin 3 , AT3 , ATX3 , JOS , MJD , MJD1 , SCA3

Cellular Localization

Nucleus matrix

Background

Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ataxin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115032

GTX115032 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Ataxin 3(GTX115032) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032

GTX115032 WB Image

Overexpression

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis.
293T mock transfected (-) or transfected with a construct expressing human Ataxin 3 (+).
10% SDS-PAGE
Ataxin 3 antibody (GTX115032) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032

GTX115032 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GTX115032) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032

GTX115032 WB Image

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and blotted with Ataxin 3 antibody (GTX115032) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032

GTX115032 WB Image

Orthogonal

The data was published in the journal Front Mol Neurosci in 2017.PMID: 28676741

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032

GTX115032 WB Image

Orthogonal

The data was published in the journal Front Mol Neurosci in 2017.PMID: 28676741

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX115032

GTX115032 WB Image

Orthogonal

The data was published in the journal Front Mol Neurosci in 2017.PMID: 28676741

REFERENCE

Application Reference

Yasaman Pakdaman et al. Front Mol Neurosci 2021; 14 : 723912 Chip Protein U-Box Domain Truncation Affects Purkinje Neuron Morphology and Leads to Behavioral Changes in Zebrafish.
Reactivity : Zebrafish
Hsu JY et al. Front Mol Neurosci 2017; 10 : 196 The Truncated C-terminal Fragment of Mutant ATXN3 Disrupts Mitochondria Dynamics in Spinocerebellar Ataxia Type 3 Models.
Application : WB
Reactivity : Mouse
Zeng LX et al. Int J Clin Exp Med 2014; 7 (4) : 973-981 Ataxin-3 expression correlates with the clinicopathologic features of gastric cancer
Application : WB
Reactivity : Human
Cho CK et al. J Proteome Res 2010; 9 (7) : 3574-82 Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Ataxin 3 antibody Cat. No. GTX115032

Rating
( Average 5 based on 1 users reviews)
Application
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Ataxin 3 antibody used in Immunoprecipitation (IP). GTX115032
Date : Anonymous submitted on 07-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HEK293T cells
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note : Antibody-bead conjugates were prepared by adding 1.0 μg of antibody to 500 μl of PBS with 0.01% triton X-100, together with 30μl of protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169