Autotaxin antibody

Name: Autotaxin antibody
Brand: GeneTex
SKU: GTX106209
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Autotaxin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106209

Anti-Autotaxin antibody used in Western Blot (WB). GTX106209

Cat. No. GTX106209

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Autotaxin products

View product citations for antibody GTX106209 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
FACS	Assay dependent

Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Autotaxin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950203

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ectonucleotide pyrophosphatase/phosphodiesterase 2 , ATX , ATX-X , AUTOTAXIN , LysoPLD , NPP2 , PD-IALPHA , PDNP2

Cellular Localization

Secreted

Background

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Gene ID: 5168 ENPP2
UniProt: Q13822 ENPP2

Research Area

DATA IMAGES

GTX106209 ICC/IF Image

Autotaxin antibody detects Autotaxin protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Autotaxin protein stained by Autotaxin antibody (GTX106209) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106209 WB Image

Human Cerebrospinal Fluid (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody (GTX106209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106209 WB Image

The data was published in the journal Sci Rep in 2020.PMID: 32111938

REFERENCE

Application Reference

Geng H et al. Am J Physiol Renal Physiol 2021; 320 (3) : F359-F374 Proximal tubule LPA1 and LPA2 receptors use divergent signaling pathways to additively increase profibrotic cytokine secretion.

Wang Z et al. J Mol Med (Berl) 2020; 98 (12) : 1781-1794 Autotaxin stimulates LPA2 receptor in macrophages and exacerbates dextran sulfate sodium-induced acute colitis.

Application : FACS

Reactivity : Human

Tsukahara T et al. Sci Rep 2020; 10 (1) : 3748 Porcine liver decomposition product-derived lysophospholipids promote microglial activation in vitro.

Application : WB

Huang YL et al. Cell Physiol Biochem 2018; 50 (2) : 597-611 High Glucose Induces VEGF-C Expression via the LPA1/3-Akt-ROS-LEDGF Signaling Axis in Human Prostate Cancer PC-3 Cells.

Application : WB

Reactivity : Human

Pleli T et al. Cell Physiol Biochem 2018; 45 (6) : 2516-2528 Activation of Adenylyl Cyclase Causes Stimulation of Adenosine Receptors.

Application : WB

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