APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human BPTF expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
bromodomain PHD finger transcription factor , FAC1 , FALZ , NEDDFL , NURF301
Cellular Localization
Cytoplasm,Nucleus
Background
This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif, suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought, and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila, the NURF complex, which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors, is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-BPTF antibody [2F10] used in ELISA (ELISA). GTX60433](/templates/default/js/lightbox/images/loading.gif "Anti-BPTF antibody [2F10] used in ELISA (ELISA). GTX60433")
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GTX60433 ELISA Image
ELISA analysis of antigen using GTX60433 BPTF antibody [2F10].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60433 WB Image
WB analysis of HEK293 (1) and BPTF (AA: 503-670)-hIgGFc transfected HEK293 (2) cell lysate using GTX60433 BPTF antibody [2F10].
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REFERENCE
There are currently no references for BPTF antibody [2F10] (GTX60433). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BPTF antibody [2F10] (GTX60433). Be the first to share your experience with this product.
![Anti-BPTF antibody [2F10] used in ELISA (ELISA). GTX60433](/upload/website/prouct_img/small/GTX60433/GTX60433_20170912_ELISA_w_23061123_742.webp "Anti-BPTF antibody [2F10] used in ELISA (ELISA). GTX60433")
![Anti-BPTF antibody [2F10] used in Western Blot (WB). GTX60433](/upload/website/prouct_img/small/GTX60433/GTX60433_20170912_WB_w_23061123_867.webp "Anti-BPTF antibody [2F10] used in Western Blot (WB). GTX60433")
![Anti-BPTF antibody [2F10] used in ELISA (ELISA). GTX60433](/upload/website/prouct_img/normal/GTX60433/GTX60433_20170912_ELISA_w_23061123_742.webp "Anti-BPTF antibody [2F10] used in ELISA (ELISA). GTX60433")
![Anti-BPTF antibody [2F10] used in Western Blot (WB). GTX60433](/upload/website/prouct_img/normal/GTX60433/GTX60433_20170912_WB_w_23061123_867.webp "Anti-BPTF antibody [2F10] used in Western Blot (WB). GTX60433")