GeneTex
United States (US)

BTK (phospho Ser180) antibody [3i5]

Cat No. GTX14393

Host Mouse
Clonality Monoclonal
Clone Name 3i5
Isotype IgG3
Application WB, IP
Reactivity Human
APPLICATION

Application Note

IP: Use at a dilution of 1/25. WB: Use at a dilution of 1/1000. Predicted molecular weight: 73 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

76 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide conjugated to KLH.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bruton Tyrosine Kinase,Agmx1,At,Atk,Bpk,Imd1,Psctk1,Xla,Btk

Cellular Localization

Cell Membrane and Cytoplasmic

Background

Brutons tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCbeta through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation. The PKC/Btk inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity.

Database

Research Area

Package List Price ($)
$ 319